PSAT1 Human

Phosphoserine Aminotransferase 1 Human Recombinant
Cat. No.
BT12733
Source
Escherichia Coli.
Synonyms
Phosphoserine aminotransferase, Phosphohydroxythreonine aminotransferase, PSAT, PSAT1, PSA, EPIP.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PSAT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 394 amino acids (1-370) and having a molecular mass of 42.9kDa.
PSAT1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Phosphoserine aminotransferase (PSAT1) is an enzyme that plays a crucial role in the biosynthesis of serine, an amino acid essential for various biological processes. PSAT1 catalyzes the conversion of 3-phosphohydroxypyruvate to 3-phosphoserine, which is then dephosphorylated to form L-serine. This enzyme exhibits high expression levels in vital organs such as the brain, liver, kidney, and pancreas, while its expression is limited in tissues like the thymus, prostate, testis, and colon. Genetic defects in the PSAT1 gene can lead to phosphoserine aminotransferase deficiency (PSATD), a disorder characterized by low serine and glycine levels in plasma and cerebrospinal fluid. PSATD manifests clinically as intractable seizures, acquired microcephaly, hypertonia, and psychomotor retardation.
Description
This product consists of the recombinant human PSAT1 protein, expressed in E. coli and purified to a high degree. The protein is a single, non-glycosylated polypeptide chain comprising 394 amino acids (residues 1-370) with a molecular weight of 42.9 kDa. A 24 amino acid His-tag is fused to the N-terminus to facilitate purification by proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The PSAT1 protein is supplied in a solution at a concentration of 1 mg/ml. The storage buffer is 20mM Tris-HCl (pH 8.0) containing 20% glycerol and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Repeated freezing and thawing should be avoided.
Purity
The purity of the PSAT1 protein is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Phosphoserine aminotransferase, Phosphohydroxythreonine aminotransferase, PSAT, PSAT1, PSA, EPIP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMDAPRQ VVNFGPGPAK LPHSVLLEIQ KELLDYKGVG ISVLEMSHRS SDFAKIINNT ENLVRELLAV PDNYKVIFLQ GGGCGQFSAV PLNLIGLKAG RCADYVVTGA WSAKAAEEAK KFGTINIVHP KLGSYTKIPD PSTWNLNPDA SYVYYCANET VHGVEFDFIP DVKGAVLVCD MSSNFLSKPV DVSKFGVIFA GAQKNVGSAG VTVVIVRDDL LGFALRECPS VLEYKVQAGN SSLYNTPPCF SIYVMGLVLE WIKNNGGAAA MEKLSSIKSQ TIYEIIDNSQ GFYVCPVEPQ NRSKMNIPFR IGNAKGDDAL EKRFLDKALE LNMLSLKGHR SVGGIRASLY NAVTIEDVQK LAAFMKKFLE MHQL.

Product Science Overview

Function and Mechanism

PSAT1 catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine, which is the second step in the de novo serine synthesis pathway . This reaction is essential for the production of serine, a non-essential amino acid that serves as a precursor for several biomolecules, including glycine, cysteine, and sphingolipids .

The enzyme requires pyridoxal-5’-phosphate (PLP) as a cofactor to facilitate the transfer of amino groups . The activity of PSAT1 is measured by its ability to produce 3-phosphooxypyruvate, with a specific activity greater than 130 pmol/min/μg under specified conditions .

Structure and Expression

Recombinant human PSAT1 is typically expressed in Escherichia coli (E. coli) and is purified with a C-terminal 6-His tag for ease of purification and detection . The recombinant protein has a predicted molecular mass of approximately 41 kDa, although it may appear as a 40 kDa band on SDS-PAGE under reducing conditions .

Clinical Significance

Mutations in the PSAT1 gene can lead to phosphoserine aminotransferase deficiency, a rare metabolic disorder characterized by low levels of serine and glycine in the blood and cerebrospinal fluid . This condition can result in severe neurological symptoms, including seizures, microcephaly, and psychomotor retardation .

Additionally, altered expression of PSAT1 has been implicated in various cancers. Overexpression of PSAT1 has been observed in certain types of cancer, suggesting that the enzyme may play a role in tumor growth and survival .

Applications in Research

Recombinant human PSAT1 is widely used in biochemical and medical research to study the serine biosynthesis pathway and its regulation . It is also utilized in the development of therapeutic strategies for conditions associated with serine deficiency and in cancer research to explore potential targets for drug development .

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