PSAP Human

Prosaposin Human Recombinant

Cat. No.

BT9449

Source

HEK 293.

Synonyms

Prosaposin, Proactivator polypeptide, PSAP, GLBA, SAP1.

Appearance

Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

PSAP Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 17-524) containing a total of 518 amino acids, having a molecular mass of 57.7kDa (calculated), though it migrates at approximately 65kDa on SDS PAGE, the PSAP is fused to a 2 a.a N-terminal linker, a 2 a.a C-terminal linker and a 6 a.a His tag at C-Terminus.
The Human PSAP is purified by proprietary chromatographic techniques.

Product Specs

Introduction

Prosaposin (PSAP) is a vital protein that serves as a precursor to four lysosomal saposin proteins (A, B, C, and D). These saposins play a crucial role in the breakdown of glycosphingolipids with short oligosaccharide chains. PSAP exhibits neurotrophic properties, promoting neurite outgrowth and enhancing choline acetyltransferase activity. It exists in both secretory and integral membrane protein forms and is found in human milk, cerebrospinal fluid, and seminal plasma, suggesting additional functions. Mutations in the PSAP gene are associated with disorders such as Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy.

Description

Recombinant Human PSAP, expressed in HEK cells, is a single, glycosylated polypeptide chain comprising 518 amino acids (a.a 17-524) with a molecular weight of 57.7 kDa (calculated). It appears as a band at approximately 65 kDa on SDS-PAGE due to glycosylation. The protein construct includes a 2 a.a N-terminal linker, a 2 a.a C-terminal linker, and a C-terminal 6 a.a His tag. Purification is achieved through proprietary chromatographic techniques.

Physical Appearance

Sterile, lyophilized powder with a white color.

Formulation

The protein was sterile filtered (0.4 µm) and lyophilized from a 0.5 mg/ml solution in phosphate buffer, pH 7.4.

Solubility

To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve a concentration of approximately 0.5 mg/ml. Allow for complete dissolution. Prior to use in cell culture, ensure sterility by filtering the solution through an appropriate sterile filter.

Stability

For long-term storage, keep the lyophilized protein at -20°C. After reconstitution, aliquot the protein and store at 4°C to minimize freeze/thaw cycles. Reconstituted PSAP remains stable for up to two weeks at 4°C without noticeable degradation.

Purity

The purity of this protein is greater than 95% as determined by SDS-PAGE analysis.

Synonyms

Prosaposin, Proactivator polypeptide, PSAP, GLBA, SAP1.

Source

HEK 293.

Amino Acid Sequence

ASGPVLGLKE CTRGSAVWCQ NVKTASDCGA VKHCLQTVWN KPTVKSLPCD ICKDVVTAAG DMLKDNATEE EILVYLEKTC DWLPKPNMSA SCKEIVDSYL PVILDIIKGE MSRPGEVCSA LNLCESLQKH LAELNHQKQL ESNKIPELDM TEVVAPFMAN IPLLLYPQDG PRSKPQPKDN GDVCQDCIQM VTDIQTAVRT NSTFVQALVE HVKEECDRLG PGMADICKNY ISQYSEIAIQ MMMHMQPKEI CALVGFCDEV KEMPMQTLVP AKVASKNVIP ALELVEPIKK HEVPAKSDVY CEVCEFLVKE VTKLIDNNKT EKEILDAFDK MCSKLPKSLS EECQEVVDTY GSSILSILLE EVSPELVCSM LHLCSGTRLP ALTVHVTQPK DGGFCEVCKK LVGYLDRNLE KNSTKQEILA ALEKGCSFLP DPYQKQCDQF VAEYEPVLIE ILVEVMDPSF VCLKIGACPS AHKPLLGTEK CIWGPSYWCQ NTETAAQCNA VEHCKRHVWN KLHHHHHH.

Product Science Overview

Structure and Function

Prosaposin is composed of 524 amino acids and has a molecular mass of approximately 57.9 kDa. The protein is synthesized as a single polypeptide chain and undergoes post-translational modifications, including glycosylation. The four saposins derived from prosaposin each have distinct roles in lipid metabolism:

Saposin A and C: These stimulate the hydrolysis of glucosylceramide and galactosylceramide by their respective enzymes.
Saposin B: This facilitates the hydrolysis of galactocerebroside sulfate, GM1 gangliosides, and globotriaosylceramide.
Saposin D: This acts as a specific activator of sphingomyelin phosphodiesterase.

Prosaposin itself functions as a myelinotrophic and neurotrophic factor, mediating its effects through G-protein-coupled receptors, GPR37 and GPR37L1.

Genetic and Clinical Significance

Mutations in the PSAP gene can lead to various lysosomal storage disorders. For instance, mutations affecting the saposin B domain cause a rare variant of metachromatic leukodystrophy (MLD), where arylsulfatase A activity remains normal. Additionally, variants in the saposin D domain have been linked to Parkinson’s disease.

Recombinant Prosaposin

Recombinant human prosaposin is produced using DNA sequences encoding the human PSAP gene, typically expressed in HEK293 cells. The recombinant protein is often tagged with polyhistidine for purification purposes and is verified for purity and activity through methods such as SDS-PAGE and HPLC.

Recombinant prosaposin is used in research to study its biological functions and potential therapeutic applications, particularly in the context of neurodegenerative diseases and lysosomal storage disorders.

Storage and Stability

Recombinant prosaposin is usually provided as a lyophilized powder and should be stored under sterile conditions at -20°C to -80°C. It is recommended to avoid repeated freeze-thaw cycles to maintain its stability and activity.

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PSAP Human

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