PRTN3 Human

Proteinase-3 Human
Cat. No.
BT26563
Source
Native.
Synonyms
AGP7, P29, PR-3, ACPA, C-ANCA, MBT, MBN, Leukocyte proteinase 3, Neutrophil proteinase 4, Wegener granulomatosis autoantigen, Azurophil granule protein 7, myeloblastin, Serine proteinase neutrophil.
Appearance
Purity

Greater than 95% in the sum of different glycosylation isoforms according to following section as determined by SDS-PAGE and capillary electrophoresis.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PRTN3 is a natural antigen having a molecular mass of 25kDa. PRTN3 is isolated from human peripheral blood leukocytes.

Product Specs

Introduction
PRTN3, also known as Proteinase 3, is a serine protease primarily found in neutrophils, a type of white blood cell. It plays a role in immune response by breaking down various proteins including elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV. Excessive PRTN3 activity has been linked to lung damage and emphysema.
Description
PRTN3 is a protein naturally present in the human body with a molecular weight of 25kDa. It is extracted from human white blood cells for research purposes.
Formulation
PRTN3 is provided dissolved in a solution containing 20mM Sodium Phosphate at pH 6.2, 300mM NaCl, and 0.02% Lubrol.
Immunological Functions
1. PRTN3 can bind to specific antibodies (IgG type) found in humans. These antibodies, called anti-PR3 antibodies, recognize and attach to particular shapes formed by PRTN3. 2. PRTN3 serves as a target for detecting these anti-PR3 antibodies through laboratory tests like ELISA and immunodot analysis.
Applications
PRTN3 can be used in Western blot experiments with specific antibodies, like rabbit anti-PR3 antisera and mouse anti-PR3 monoclonal antibodies, to detect its presence.
Coating Concentration
Depending on the experimental setup (ELISA plate type and coating buffer), a concentration of 0.5-1.0 µg/ml of PRTN3 is suitable for coating surfaces. This coating allows for the attachment of other molecules or cells for various assays.
Purity
The purity of PRTN3 is greater than 95%, considering all its different forms (glycosylation isoforms). This purity level is determined using SDS-PAGE and capillary electrophoresis, which are techniques used to separate and analyze proteins.
Stability
To maintain product stability, store PRTN3 at 4°C if using within 2-4 weeks. For long-term storage, freeze at -20°C. Minimize repeated freezing and thawing to preserve product integrity.
Synonyms
AGP7, P29, PR-3, ACPA, C-ANCA, MBT, MBN, Leukocyte proteinase 3, Neutrophil proteinase 4, Wegener granulomatosis autoantigen, Azurophil granule protein 7, myeloblastin, Serine proteinase neutrophil.
Source
Native.

Product Science Overview

Gene and Expression

The PRTN3 gene is located on chromosome 19 at band 19p13.3 . The gene’s expression is predominantly found in bone marrow, blood monocytes, and granulocytes . In neutrophils, PR3 is stored in azurophil granules and is released during the immune response .

Function

PR3 is involved in the proteolytic generation of antimicrobial peptides, which are essential for the body’s defense against infections . It contributes to the degradation of elastin and other extracellular matrix proteins, aiding in tissue remodeling and repair . Additionally, PR3 plays a role in the regulation of inflammation and immune responses .

Clinical Significance

PR3 is the target of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cytoplasmic subtype (c-ANCA) . These antibodies are frequently found in patients with granulomatosis with polyangiitis (GPA), an autoimmune vasculitis that affects small to medium-sized blood vessels . The presence of PR3-ANCA is a specific diagnostic marker for GPA and is used in clinical settings to differentiate it from other forms of vasculitis .

Research and Applications

PR3 is used in immunoassays to detect anti-PR3 autoantibodies, which are indicative of autoimmune conditions like GPA . Research continues to explore the exact role of PR3 in neutrophil function and its potential implications in other diseases .

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