PRSS3 Human, HEK
HEK293 Cells.
Protease, Serine, 3, Protease, Serine, 4 (Trypsin 4, Brain), Brain Trypsinogen, Mesotrypsinogen, Mesotrypsin, Trypsin III, EC 3.4.21.4 4, Trypsin IV, PRSS4, TRY3, TRY4 Protease, Serine, 3 (Mesotrypsin), Pancreatic Trypsinogen III, Serine Protease 3, Serine Protease 4, Trypsinogen IV, Trypsinogen 4, Trypsinogen 5, EC 3.4.21, MTG, T9.
Greater than 95.0% as determined by SDS-PAGE.
Description
PRSS3 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 238 amino acids (16-247 a.a.) and having a molecular mass of 26kDa. PRSS3 is fused to a 6 amino acid His-tag at C-terminus and is purified by proprietary chromatographic techniques.
Product Specs
Protease, Serine, 3, Protease, Serine, 4 (Trypsin 4, Brain), Brain Trypsinogen, Mesotrypsinogen, Mesotrypsin, Trypsin III, EC 3.4.21.4 4, Trypsin IV, PRSS4, TRY3, TRY4 Protease, Serine, 3 (Mesotrypsin), Pancreatic Trypsinogen III, Serine Protease 3, Serine Protease 4, Trypsinogen IV, Trypsinogen 4, Trypsinogen 5, EC 3.4.21, MTG, T9.
HEK293 Cells.
VPFDDDDKIV GGYTCEENSL PYQVSLNSGS HFCGGSLISE QWVVSAAHCY KTRIQVRLGE HNIKVLEGNE QFINAAKIIR HPKYNRDTLD NDIMLIKLSS PAVINARVST ISLPTAPPAA GTECLISGWG NTLSFGADYP DELKCLDAPV LTQAECKASY PGKITNSMFC VGFLEGGKDS CQRDSGGPVV CNGQLQGVVS WGHGCAWKNR PGVYTKVYNY VDWIKDTIAA NSHHHHHH.
Product Science Overview
PRSS3 is a single, glycosylated polypeptide chain consisting of 238 amino acids, with a molecular mass of approximately 26 kDa . The recombinant form of PRSS3 produced in HEK293 cells is fused to a 6 amino acid His-tag at the C-terminus, which facilitates its purification through chromatographic techniques .
As a serine protease, PRSS3 plays a crucial role in the hydrolysis of peptide bonds in proteins, a process essential for protein digestion and metabolism. The enzyme operates by cleaving peptide bonds at the carboxyl side of lysine and arginine residues. This activity is vital for various physiological processes, including the activation of other proteases and the regulation of cellular functions.
PRSS3 is predominantly expressed in the pancreas, where it is secreted as an inactive zymogen (trypsinogen) and later activated in the small intestine. However, its expression is not limited to the pancreas. Studies have shown that PRSS3 is also expressed in other tissues such as the liver, heart, and testis, as well as in several tumor cell lines .
The activity of PRSS3 is significant in the context of various diseases. For instance, abnormal expression or mutations in the PRSS3 gene have been linked to pancreatic disorders and certain types of cancer. The enzyme’s ability to degrade fibrinogen and activate pro-urokinase-type plasminogen activator (pro-uPA) suggests its potential role in fibrinolysis and tumor progression .
The production of recombinant PRSS3 in HEK293 cells offers several advantages. HEK cells provide a human-like post-translational modification system, ensuring that the recombinant protein is correctly folded and glycosylated. This is crucial for maintaining the enzyme’s activity and stability. The use of a His-tag facilitates the purification process, allowing for the isolation of high-purity PRSS3 for research and therapeutic applications .
