PRSS28 Mouse

PRSS28 is synthesized as a proenzyme (pro-form) and requires activation to become fully functional. The recombinant form of PRSS28 is typically produced in a mouse myeloma cell line (NS0) and includes a C-terminal 10-His tag for purification purposes . The protein has a predicted molecular mass of approximately 30 kDa, but it often appears as a 34 kDa band under reducing conditions in SDS-PAGE due to post-translational modifications .

PRSS28 is co-expressed with another serine protease, ISP2/Prss29, in pre-implantation embryos and the uterus . It closely resembles mouse mast cell tryptases, specifically MCP-6 and MCP-7 . The enzyme exhibits mixed substrate specificity, which allows it to silence signaling through proteinase-activated receptors .

Recombinant PRSS28 is used in various biochemical assays to study its enzymatic activity. It is measured by its ability to cleave specific peptide substrates, such as Suc-VPF-SBzl, after activation by Thermolysin . The specific activity of PRSS28 is greater than 15,000 pmol/min/µg under the described conditions .

The recombinant protein is supplied as a 0.2 μm filtered solution in Tris and NaCl and should be stored at -70°C to maintain its stability . It is important to avoid repeated freeze-thaw cycles to preserve its activity.