PRSS22 Mouse

PRSS22 is produced in Sf9 Baculovirus cells and is a single, glycosylated polypeptide chain containing 283 amino acids (33-307) with a molecular mass of approximately 31.1 kDa . The recombinant form of this protein is fused to an 8 amino acid His-Tag at the C-terminus, which facilitates its purification through chromatographic techniques .

The amino acid sequence of PRSS22 includes several key regions that contribute to its function as a serine protease. The protein solution is typically formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol to maintain stability .

PRSS22 plays a crucial role in various biological processes due to its serine-type endopeptidase activity. This activity is essential for the breakdown of peptide bonds in proteins, which is a critical function in numerous physiological processes . The enzyme’s expression in the brain and other epithelium-rich tissues suggests it may have specialized roles in these areas, although the full extent of its functions is still being explored.

Recombinant PRSS22 is widely used in laboratory research to study its enzymatic properties and potential roles in various diseases. For instance, it has been associated with conditions such as prostate cancer and Hermansky-Pudlak Syndrome . Researchers utilize recombinant forms of PRSS22 to investigate its structure-function relationships, substrate specificity, and potential as a therapeutic target.

For optimal stability, PRSS22 should be stored at 4°C if used within 2-4 weeks. For longer-term storage, it is recommended to freeze the protein at -20°C and add a carrier protein such as 0.1% HSA or BSA to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .