PRPSAP2 Human

Phosphoribosyl Pyrophosphate Synthetase-Associated Protein 2 Human Recombinant

Cat. No.

BT9294

Source

Escherichia Coli.

Synonyms

PAP41, Phosphoribosyl pyrophosphate synthase-associated protein 2, PRPP synthase-associated protein 2, 41 kDa phosphoribosypyrophosphate synthetase-associated protein, PRPSAP2.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

PRPSAP2 Human Recombinant produced in E. coli is a single polypeptide chain containing 392 amino acids (1-369) and having a molecular mass of 43.3kDa. PRPSAP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Phosphoribosyl Pyrophosphate Synthetase-Associated Protein 2 (PRPSAP2) is a protein that interacts with the enzyme phosphoribosylpyrophosphate synthetase (PRS). PRS is responsible for catalyzing the creation of phosphoribosylpyrophosphate, a crucial component in the synthesis of purine, pyrimidine, histidine, tryptophan, and NAD. PRS functions as a complex consisting of two catalytic subunits and two associated subunits. PRPSAP2 encodes one of these non-catalytic associated subunits of PRS.

Description

Recombinant human PRPSAP2, produced in E. coli, is a single polypeptide chain consisting of 392 amino acids (residues 1-369) with a molecular weight of 43.3kDa. The PRPSAP2 protein includes a 23 amino acid His-tag fused to the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

A clear solution that has undergone sterile filtration.

Formulation

The PRPSAP2 solution is provided at a concentration of 0.25mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.

Stability

For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.

Purity

Purity is determined to be greater than 90% based on SDS-PAGE analysis.

Synonyms

PAP41, Phosphoribosyl pyrophosphate synthase-associated protein 2, PRPP synthase-associated protein 2, 41 kDa phosphoribosypyrophosphate synthetase-associated protein, PRPSAP2.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMFCVTPP ELETKMNITK GGLVLFSANS NSSCMELSKK IAERLGVEMG KVQVYQEPNR ETRVQIQESV RGKDVFIIQT VSKDVNTTIM ELLIMVYACK TSCAKSIIGV IPYFPYSKQC KMRKRGSIVS KLLASMMCKA GLTHLITMDL HQKEIQGFFN IPVDNLRASP FLLQYIQEEI PDYRNAVIVA KSPASAKRAQ SFAERLRLGI AVIHGEAQDA ESDLVDGRHS PPMVRSVAAI HPSLEIPMLI PKEKPPITVV GDVGGRIAII VDDIIDDVDS FLAAAETLKE RGAYKIFVMA THGLLSSDAP RRIEESAIDE VVVTNTIPHE VQKLQCPKIK TVDISMILSE AIRRIHNGES MSYLFRNIGL DD.

Product Science Overview

Gene and Protein Information

Gene Name: PRPSAP2
Protein Name: Phosphoribosyl Pyrophosphate Synthetase-Associated Protein 2
Aliases: PAP41, PRPP Synthase-Associated Protein 2
Gene ID: 5636 (NCBI)
UniProt ID: O60256

Biological Role

PRPSAP2 is involved in the formation of phosphoribosyl pyrophosphate (PRPP), a substrate essential for the synthesis of purine and pyrimidine nucleotides, as well as the amino acids histidine and tryptophan, and the coenzyme NAD. PRPSAP2 forms a complex with two catalytic subunits and two associated subunits of PRS.

Function and Mechanism

PRPSAP2 is believed to play a negative regulatory role in the synthesis of 5-phosphoribose 1-diphosphate. This regulation is crucial for maintaining the balance of nucleotide synthesis within the cell. The protein’s function is associated with magnesium ion binding and ribose phosphate diphosphokinase activity.

Clinical Significance

Mutations or dysregulation of the PRPSAP2 gene have been linked to several disorders, including Intellectual Developmental Disorder, Autosomal Recessive 71, and Arts Syndrome. These conditions highlight the importance of PRPSAP2 in normal cellular function and development.

Research and Applications

Recombinant forms of PRPSAP2 are used in research to study its function and regulatory mechanisms. Understanding the role of PRPSAP2 can provide insights into the metabolic pathways involved in nucleotide synthesis and potential therapeutic targets for related disorders.

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PRPSAP2 Human

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