PRPS1 Antibody

Phosphoribosyl Pyrophosphate Synthetase 1, Mouse Anti Human
Cat. No.
BT24476
Source
Synonyms
ARTS, CMTX5, PPRibP, PRSI, DFN2, Ribose-phosphate pyrophosphokinase 1, DFNX1, Phosphoribosyl pyrophosphate synthase I, PRS-I, PRPS1, KIAA0967.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
PRPS1, an enzyme crucial for nucleotide synthesis, catalyzes the formation of phosphoribosylpyrophosphate (PRPP) from ribose 5-phosphate. This process is essential for purine metabolism and nucleotide biosynthesis. Defects in PRPS1 can lead to various conditions, including phosphoribosylpyrophosphate synthetase superactivity, Charcot-Marie-Tooth disease X-linked recessive type 5, and Arts Syndrome.
Physical Appearance
A clear, colorless solution that has been sterilized through filtration.
Formulation
The solution is provided at a concentration of 1mg/ml and contains the following components: PBS (pH 7.4), 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), the solution should be kept at 4°C. For longer storage periods, it is recommended to store the solution at -20°C. To maintain product integrity, avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The solution remains stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
This antibody has undergone rigorous testing using ELISA and Western blot analysis to confirm its specificity and reactivity. However, optimal working dilutions may vary based on the specific application. Therefore, users are advised to perform their own titrations to determine the most suitable dilution for their experiments. A starting dilution of 1:1000 is recommended.
Synonyms
ARTS, CMTX5, PPRibP, PRSI, DFN2, Ribose-phosphate pyrophosphokinase 1, DFNX1, Phosphoribosyl pyrophosphate synthase I, PRS-I, PRPS1, KIAA0967.
Purification Method
PRPS1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1E11AT.
Immunogen
Anti-human PRPS1 mAb, clone PAT1E11AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PRPS1 protein 1-318 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.

Product Science Overview

Mouse Anti-Human Antibodies

Mouse anti-human antibodies are secondary antibodies produced by immunizing mice with human antigens. These antibodies are commonly used in various laboratory techniques, including enzyme-linked immunosorbent assays (ELISA), Western blotting, and immunohistochemistry .

Production and Applications

Mouse anti-human antibodies are generated by injecting mice with human immunoglobulins or other human proteins. The mice then produce antibodies against these human proteins, which are harvested and purified for use in research and diagnostic applications . These secondary antibodies are often conjugated with enzymes like horseradish peroxidase (HRP) or fluorescent dyes to facilitate detection and quantification of target proteins .

Clinical Relevance

One of the challenges associated with the use of mouse anti-human antibodies is the potential for the human anti-mouse antibody (HAMA) response. This immune response can occur when human patients develop antibodies against the mouse-derived antibodies, leading to allergic reactions and potentially interfering with diagnostic tests .

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