Mouse anti-human antibodies are secondary antibodies produced by immunizing mice with human antigens. These antibodies are commonly used in various laboratory techniques, including enzyme-linked immunosorbent assays (ELISA), Western blotting, and immunohistochemistry .
Mouse anti-human antibodies are generated by injecting mice with human immunoglobulins or other human proteins. The mice then produce antibodies against these human proteins, which are harvested and purified for use in research and diagnostic applications . These secondary antibodies are often conjugated with enzymes like horseradish peroxidase (HRP) or fluorescent dyes to facilitate detection and quantification of target proteins .
One of the challenges associated with the use of mouse anti-human antibodies is the potential for the human anti-mouse antibody (HAMA) response. This immune response can occur when human patients develop antibodies against the mouse-derived antibodies, leading to allergic reactions and potentially interfering with diagnostic tests .