Protein-A/G/L
Protein A/G/L Recombinant
Cat. No.
BT9494
Source
Escherichia Coli.
Synonyms
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Recombinant Protein-A/G/L produced in E.Coli is a single non-glycosylated polypeptide chain. Protein- A/G/L is comprised of 5 IgG-binding regions of Protein A (E-D-A-B-C), 2 of protein G (C1-C3) and 5 of Protein L (B1-B2-B3-B4-B5) containing 805 amino acids in total and having a molecular mass of 89.2kDa. Cell wall binding region, cell membrane binding region and albumin binding region have been eliminated from the recombinant Protein- A/G/L to guarantee the maximum specific IgG binding.
Product Specs
Introduction
Protein A/G/L is a recombinant protein engineered for its IgG binding capabilities, encompassing the binding profiles of Protein A, Protein G, and Protein L. This gene fusion product comprises five Ig-binding regions from Protein L (B1-B2-B3-B4-B5), five IgG binding domains from Protein A (E-D-A-B-C), and two Ig-binding regions from Protein G (C1-C3). It serves as a versatile tool for purifying monoclonal and polyclonal IgG antibodies, demonstrating binding affinity for IgG derived from various species, including humans, mice, rats, cows, goats, sheep, rabbits, guinea pigs, pigs, dogs, and cats.
Description
Recombinant Protein-A/G/L, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 805 amino acids, encompassing five IgG-binding regions from Protein A (E-D-A-B-C), two from Protein G (C1-C3), and five from Protein L (B1-B2-B3-B4-B5). Its molecular mass is 89.2kDa. The recombinant Protein-A/G/L is designed for optimal IgG binding specificity, achieved by eliminating the cell wall binding region, cell membrane binding region, and albumin binding region.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Protein- A/G/L is lyophilized without the addition of any other substances.
Solubility
To reconstitute lyophilized Protein-A/G/L, it is recommended to dissolve it in sterile 18M-cm H2O at a minimum concentration of 0.1mg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Protein-A/G/L remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, Protein-A/G/L should be stored at 4°C for 2-7 days. For extended storage, it is advisable to store it below -18°C. Adding a carrier protein, such as 0.1% HSA or BSA, is recommended for long-term storage. Repeated freezing and thawing of the protein should be avoided.
Purity
The purity of this protein is greater than 95.0%, as determined by SEC-HPLC analysis and SDS-PAGE analysis.
Source
Escherichia Coli.
Amino Acid Sequence
NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEE PRARPGSGSG KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAG.
Product Science Overview
Components and Origins
-
Protein A:
- Source: Originally derived from the bacterium Staphylococcus aureus.
- Binding Specificity: Binds primarily to the Fc region of IgG antibodies, particularly those from humans, rabbits, pigs, dogs, and cats .
- Structure: Contains five IgG-binding domains, each capable of binding to the Fc region of an antibody .
-
Protein G:
-
Protein L:
Recombinant Fusion Protein
The recombinant fusion protein A/G/L combines the Ig-binding domains of Protein A, Protein G, and Protein L. This fusion protein is expressed in Escherichia coli and has been engineered to eliminate non-specific binding regions, such as cell wall binding regions and albumin binding regions, to ensure maximum specific IgG binding .
Applications
-
Antibody Purification:
-
Immunoprecipitation:
-
Diagnostic and Therapeutic Research:
Advantages
- Versatility: The combination of binding domains from Protein A, Protein G, and Protein L allows Protein A/G/L to bind a wide range of antibodies from different species and subclasses .
- Specificity: The elimination of non-specific binding regions ensures high specificity in antibody binding and purification .
- Efficiency: The recombinant nature of Protein A/G/L allows for consistent and efficient production, making it a reliable tool in research and clinical applications .
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