Prolactin Chicken
Greater than 95.0% as determined by gel filtration analysis.
Description
Prolactin Chicken Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids + an additional Ala at n-terminal and having a molecular mass of ~ 23 kDa.
The Chicken Prolactin protein is purified by proprietary chromatographic techniques.
Product Specs
Prolactin, a neuroendocrine hormone primarily produced by the pituitary gland, is also found in other cells like those in the placenta, brain, and uterus. Its secretion is triggered by various stimuli such as eating, nursing, mating, estrogen treatment, and ovulation. Prolactin's main function is to stimulate and maintain milk production (lactation). Additionally, it plays a role in breast cancer development, reproductive function regulation, and immune system modulation.
Recombinant Chicken Prolactin, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 199 amino acids, with an additional alanine residue at the N-terminus. Its molecular weight is approximately 23 kDa.
Purification of the Chicken Prolactin protein is achieved through proprietary chromatographic methods.
The protein was lyophilized from a 1 mg/ml solution containing 0.02% sodium bicarbonate (NaHCO3).
To reconstitute the lyophilized Prolactin, it is recommended to use sterile 0.4% NaHCO3 at pH 8.5, ensuring a concentration of at least 100 µg/ml. This solution can be further diluted with other aqueous solutions as needed.
While lyophilized Prolactin remains stable at room temperature for up to 3 weeks, it should be stored desiccated at a temperature below -18°C. After reconstitution, Prolactin should be stored at 4°C for 2-7 days. For long-term storage, freeze at -18°C.
Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage.
Avoid repeated freeze-thaw cycles.
The purity of the protein is determined to be greater than 95.0% using gel filtration analysis.
The biological activity of Chicken Prolactin was assessed in vitro by evaluating its ability to induce proliferation in Nb2 cells and Baf/3 cells stably transfected with Chicken Prolactin receptors.
The a.a. sequence of the first 6 N-terminal a.a was found to be Ala-Leu-Pro-Ile-Cys-Pro.
Product Science Overview
Prolactin is composed of a single-chain polypeptide with approximately 199 amino acids. The hormone’s structure allows it to bind to specific prolactin receptors (PRLR) on target cells, initiating a cascade of intracellular events that lead to its diverse physiological effects. In chickens, prolactin is involved in the regulation of incubation behavior, hormone secretion, and follicular development .
Recombinant prolactin refers to prolactin that is produced through recombinant DNA technology. This involves inserting the gene encoding chicken prolactin into a suitable expression system, such as bacteria or yeast, to produce the hormone in large quantities. Recombinant prolactin is used in various research and commercial applications, including studies on reproductive physiology and the development of poultry breeding programs.
In poultry, prolactin is essential for the onset of broodiness, a behavior characterized by the desire to incubate eggs and care for chicks. This hormone’s levels increase significantly during the breeding season, promoting nesting and hatching behaviors. Additionally, prolactin influences the secretion of other reproductive hormones and plays a role in the development of the reproductive system .
Prolactin also exhibits important roles in the immune system maintenance. It is involved in the regulation of the nervous system, internal environment stability, and various body functional activities. In poultry, prolactin and growth hormone (GH) are related to growth, development, and reproduction. Some studies have shown that after chickens are infected with certain pathogens, the hormone levels change, and they are involved in regulating the body’s immune response .
The study of recombinant prolactin has provided valuable insights into its functions and potential applications. Researchers have explored its role in various physiological processes, including its impact on growth, development, and immune response. Recombinant prolactin is also used in the development of poultry breeding programs, where it helps improve reproductive efficiency and overall flock health.
In conclusion, prolactin (chicken recombinant) is a vital hormone with diverse roles in reproduction, metabolism, and immune regulation. Its study and application in poultry science have contributed significantly to our understanding of avian physiology and the development of more efficient breeding programs.
