PRMT3 Human
Protein Arginine Methyltransferase 3 Human Recombinant
Cat. No.
BT12538
Source
Escherichia Coli.
Synonyms
Protein Arginine Methyltransferase 3, Heterogeneous Nuclear Ribonucleoprotein, Methyltransferase-Like Protein 3, HRMT1L3, HMT1 HnRNP Methyltransferase-Like 3 (S. Cerevisiae), Protein Arginine N-Methyltransferase 3, HMT1 HnRNP Methyltransferase-Like 3, EC 2.1.1.- ,EC 2.1.1, PRMT3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PRMT3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 554 amino acids (1-531 a.a) and having a molecular mass of 62.3kDa.
PRMT3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PRMT3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
PRMT3, also known as protein arginine N-methyltransferase 3, is a member of the protein arginine methyltransferase family. It catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. PRMT3 operates on 40S ribosomal protein S2, rpS2, which is the major in-vivo substrate. Additionally, PRMT3 is also involved in the proper maturation of the 80S ribosome.
Description
Recombinant human PRMT3 protein was expressed in E. coli as a non-glycosylated polypeptide chain with a molecular mass of 62.3 kDa. The protein contains 554 amino acids (1-531 a.a) and is fused to a 23 amino acid His-tag at the N-terminus. The protein was purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The PRMT3 protein solution is provided at a concentration of 0.5 mg/ml in phosphate-buffered saline (pH 7.4) containing 20% glycerol and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Protein Arginine Methyltransferase 3, Heterogeneous Nuclear Ribonucleoprotein, Methyltransferase-Like Protein 3, HRMT1L3, HMT1 HnRNP Methyltransferase-Like 3 (S. Cerevisiae), Protein Arginine N-Methyltransferase 3, HMT1 HnRNP Methyltransferase-Like 3, EC 2.1.1.- ,EC 2.1.1, PRMT3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMCSLASG ATGGRGAVEN EEDLPELSDS GDEAAWEDED DADLPHGKQQ TPCLFCNRLF TSAEETFSHC KSEHQFNIDS MVHKHGLEFY GYIKLINFIR LKNPTVEYMN SIYNPVPWEK EEYLKPVLED DLLLQFDVED LYEPVSVPFS YPNGLSENTS VVEKLKHMEA RALSAEAALA RAREDLQKMK QFAQDFVMHT DVRTCSSSTS VIADLQEDED GVYFSSYGHY GIHEEMLKDK IRTESYRDFI YQNPHIFKDK VVLDVGCGTG ILSMFAAKAG AKKVLGVDQS EILYQAMDII RLNKLEDTIT LIKGKIEEVH LPVEKVDVII SEWMGYFLLF ESMLDSVLYA KNKYLAKGGS VYPDICTISL VAVSDVNKHA DRIAFWDDVY GFKMSCMKKA VIPEAVVEVL DPKTLISEPC GIKHIDCHTT SISDLEFSSD FTLKITRTSM CTAIAGYFDI YFEKNCHNRV VFSTGPQSTK THWKQTVFLL EKPFSVKAGE ALKGKVTVHK NKKDPRSLTV TLTLNNSTQT YGLQ
Product Science Overview
Classification and Structure
PRMTs are classified into three main types based on the type of methylation they catalyze:
- Type I PRMTs: Catalyze the formation of monomethylarginine (MMA) and asymmetric dimethylarginine (ADMA). This group includes PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, and PRMT8.
- Type II PRMTs: Catalyze the formation of monomethylarginine (MMA) and symmetric dimethylarginine (SDMA). This group includes PRMT5 and PRMT9.
- Type III PRMTs: Catalyze only the formation of monomethylarginine (MMA). PRMT7 is the sole member of this group .
PRMT3 specifically catalyzes the formation of MMA and ADMA, classifying it as a Type I PRMT. Structurally, PRMT3 contains a conserved catalytic core that binds S-adenosyl-L-methionine (SAM), the methyl donor in the methylation reaction .
Biological Properties and Functions
PRMT3 is involved in several critical biological processes:
- Ribosome Biogenesis: PRMT3 methylates the 40S ribosomal protein S2 (rpS2), which is essential for the proper maturation of the 80S ribosome .
- Gene Expression Regulation: Through its methylation activity, PRMT3 influences the expression of various genes by modifying histones and other transcriptional regulators .
- Signal Transduction: PRMT3-mediated methylation of signaling proteins can modulate their activity and interactions, thereby affecting various signaling pathways .
Modes of Action
PRMT3 exerts its effects primarily through the methylation of arginine residues on target proteins. This methylation can alter the protein’s function, stability, localization, and interactions with other molecules. For example, the methylation of rpS2 by PRMT3 is crucial for ribosome assembly and function .
Regulatory Mechanisms
The activity of PRMT3 is regulated at multiple levels:
- Transcriptional Regulation: The expression of the PRMT3 gene can be modulated by various transcription factors and signaling pathways.
- Post-translational Modifications: PRMT3 itself can be subject to post-translational modifications, such as phosphorylation, which can influence its activity and stability.
- Protein-Protein Interactions: PRMT3 can interact with other proteins, which can modulate its substrate specificity and catalytic activity .
Clinical Relevance
PRMT3 has been implicated in various diseases, particularly cancer. Overexpression of PRMT3 has been observed in certain types of cancer, and it is thought to contribute to tumorigenesis by promoting the methylation of oncogenic proteins and altering gene expression patterns . As a result, PRMT3 is considered a potential target for therapeutic intervention in cancer treatment .
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