PRKRA Human

Protein Kinase IFN Double Stranded RNA Activator Human Recombinant

Cat. No.

BT14443

Source

Escherichia Coli.

Synonyms

Interferon-inducible double-stranded RNA-dependent protein kinase activator A, Protein Kinase IFN Double Stranded RNA Activator, PKR-associated protein X, PKR-associating protein X, Protein activator of the interferon-induced protein kinase, Protein kinase, interferon-inducible double-stranded RNA-dependent activator, PRKRA, PACT, RAX, HSD-14, HSD14, Protein kinase, interferon-inducible double stranded RNA dependent activator, DYT16.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs

In Stock

Quick Inquiry

Description

PRKRA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 336 amino acids (1-313a.a) and having a molecular mass of 36.8kDa.
PRKRA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Protein Kinase R (PKR) Activator, also known as PRKRA, is a protein involved in the cellular response to viral infections. Upon viral infection, PRKRA activates PKR even without the presence of double-stranded RNA (dsRNA), which is typically a hallmark of viral presence. This activation leads to the phosphorylation of eukaryotic initiation factor 2 alpha (eIF2α), ultimately inhibiting protein synthesis and triggering apoptosis, both of which are crucial for controlling viral spread. Mutations in the PRKRA gene have been linked to dystonia, a neurological disorder characterized by involuntary muscle contractions.

Description

This product consists of the human recombinant PRKRA protein, produced in E. coli. It is a single, non-glycosylated polypeptide chain composed of 336 amino acids (specifically, amino acids 1 through 313 of the native protein with an additional 23-amino acid Histidine tag at the N-terminus). The molecular weight of the recombinant protein is 36.8 kDa. The protein has undergone purification using proprietary chromatographic methods.

Physical Appearance

A clear and colorless solution that has been sterilized by filtration.

Formulation

The PRKRA protein is provided in a solution at a concentration of 0.25 mg/ml. The solution contains the following components: 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT, and 1mM EDTA.

Stability

For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, it is advisable to add a carrier protein such as albumin (HSA or BSA) to a final concentration of 0.1%. It is crucial to minimize repeated freeze-thaw cycles to maintain protein integrity.

Purity

The purity of the PRKRA protein is greater than 80%, as determined by SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSQSRHR AEAPPLERED SGTFSLGKMI TAKPGKTPIQ VLHEYGMKTK NIPVYECERS DVQIHVPTFT FRVTVGDITC TGEGTSKKLA KHRAAEAAIN ILKANASICF AVPDPLMPDP SKQPKNQLNP IGSLQELAIH HGWRLPEYTL SQEGGPAHKR EYTTICRLES FMETGKGASK KQAKRNAAEK FLAKFSNISP ENHISLTNVV GHSLGCTWHS LRNSPGEKIN LLKRSLLSIP NTDYIQLLSE IAKEQGFNIT YLDIDELSAN GQYQCLAELS TSPITVCHGS GISCGNAQSD AAHNALQYLK IIAERK.

Product Science Overview

Introduction

Protein Kinase RNA-activated (PKR) is a crucial enzyme in the human body, encoded by the EIF2AK2 gene on chromosome 2. It is a serine/threonine kinase that plays a significant role in the cellular response to viral infections and other stress signals. PKR is activated by double-stranded RNA (dsRNA), which is often produced during viral replication. This activation leads to the phosphorylation of the eukaryotic translation initiation factor 2 alpha (eIF2α), resulting in the inhibition of protein synthesis and the suppression of viral replication.

Mechanism of Action

PKR is primarily activated by binding to dsRNA, which induces its autophosphorylation and subsequent activation. Once activated, PKR phosphorylates eIF2α, leading to a reduction in protein synthesis. This mechanism is a part of the cell’s antiviral defense, as it helps to limit the production of viral proteins . Additionally, PKR can interact with other cellular proteins, such as PACT (Protein Activator of the Interferon-induced Protein Kinase), which further modulates its activity.

Role in Viral Infections

PKR plays a pivotal role in the cellular response to viral infections. For instance, during HIV-1 replication, PKR is transiently activated, leading to the phosphorylation of eIF2α and a decrease in viral protein synthesis. However, HIV-1 has evolved mechanisms to counteract this response. The virus can modulate the activity of PKR through various viral and cellular factors, including the interaction with PACT and ADAR1 (Adenosine Deaminase Acting on RNA), which can inhibit PKR activity and promote viral replication.

Therapeutic Potential

Given its crucial role in the antiviral response, PKR is a potential target for therapeutic interventions. Modulating PKR activity could enhance the body’s ability to fight viral infections. For example, enhancing PKR activation could improve the antiviral response, while inhibiting PKR could be beneficial in conditions where excessive PKR activity leads to detrimental effects, such as in certain inflammatory diseases.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Product Name

Quantity&Size*

Message

PRKRA Human

Description

Product Specs

Product Science Overview

Quick Inquiry

Category

Service