PRKAR2A Human

Protein Kinase CAMP-Dependent Regulatory Type II Alpha Human Recombinant
Cat. No.
BT14379
Source
Escherichia Coli.
Synonyms
cAMP-dependent protein kinase type II-alpha regulatory subunit, PKR2, PRKAR2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PRKAR2A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 434 amino acids (1-404 a.a) and having a molecular mass of 48.6kDa.
PRKAR2A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The regulatory subunit of cAMP-dependent protein kinase type II-alpha, known as PRKAR2A, is a target for phosphorylation by the activated catalytic subunit. It interacts with various A-kinase anchoring proteins, playing a role in regulating the localization of cAMP-dependent protein kinase within the cell.
Description
Recombinant PRKAR2A, of human origin, is produced in E. coli. This results in a single, non-glycosylated polypeptide chain consisting of 434 amino acids (specifically, amino acids 1 to 404). The protein has a molecular mass of 48.6 kDa. A 23 amino acid His-tag is fused to the N-terminus of PRKAR2A. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
The solution is sterile, filtered, and colorless.
Formulation
The PRKAR2A protein solution has a concentration of 0.5 mg/ml. It is prepared in a phosphate-buffered saline solution with a pH of 7.4. The solution also contains 10% glycerol and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For longer storage, it's recommended to freeze the solution at -20°C. Adding a carrier protein like 0.1% HSA or BSA is advisable for long-term storage. It's important to minimize freeze-thaw cycles.
Purity
Analysis by SDS-PAGE indicates a purity greater than 85.0%.
Synonyms
cAMP-dependent protein kinase type II-alpha regulatory subunit, PKR2, PRKAR2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSHIQIP PGLTELLQGY TVEVLRQQPP DLVEFAVEYF TRLREARAPA SVLPAATPRQ SLGHPPPEPG PDRVADAKGD SESEEDEDLE VPVPSRFNRR VSVCAETYNP DEEEEDTDPR VIHPKTDEQR CRLQEACKDI LLFKNLDQEQ LSQVLDAMFE RIVKADEHVI DQGDDGDNFY VIERGTYDIL VTKDNQTRSV GQYDNRGSFG ELALMYNTPR AATIVATSEG SLWGLDRVTF RRIIVKNNAK KRKMFESFIE SVPLLKSLEV SERMKIVDVI GEKIYKDGER IITQGEKADS FYIIESGEVS ILIRSRTKSN KDGGNQEVEI ARCHKGQYFG ELALVTNKPR AASAYAVGDV KCLVMDVQAF ERLLGPCMDI MKRNISHYEE QLVKMFGSSV DLGNLGQ.

Product Science Overview

Structure and Function

The PKA holoenzyme is a tetramer composed of two regulatory ® subunits and two catalytic © subunits. The regulatory subunits bind to cAMP, leading to the dissociation of the holoenzyme into a dimer of regulatory subunits bound to four cAMP molecules and two free monomeric catalytic subunits . This dissociation activates the catalytic subunits, which then phosphorylate various target proteins to elicit cellular responses.

PRKAR2A is one of the four regulatory subunits identified in humans. It can be phosphorylated by the activated catalytic subunit and interacts with various A-kinase anchoring proteins (AKAPs), determining the subcellular localization of PKA . This subunit is involved in regulating protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER) .

Biological Significance

cAMP-dependent protein kinase is essential for numerous cellular functions, including:

  • Regulation of glycogen, sugar, and lipid metabolism
  • Control of cell cycle and cell division
  • Modulation of gene expression
  • Regulation of ion channel conductivity
  • Influence on neurotransmitter release and synaptic plasticity

PRKAR2A, specifically, has been shown to regulate protein transport and is crucial for proper cellular signaling and function .

Clinical Relevance

Mutations or dysregulation of PRKAR2A have been associated with various diseases, including:

  • Carney Complex Variant: A condition characterized by multiple neoplasms and skin abnormalities .
  • Macs Syndrome: A rare genetic disorder affecting multiple systems .

Additionally, PRKAR2A is involved in pathways related to anti-inflammatory cytokine production and beta-2 adrenergic-dependent CFTR expression .

Research and Applications

Recombinant human PRKAR2A is widely used in research to study its role in cellular signaling and disease mechanisms. Understanding the function and regulation of this protein can provide insights into developing therapeutic strategies for related diseases.

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