PRKAG1 Human
Protein Kinase, AMP-Activated, Gamma 1 Human Recombinant
Cat. No.
BT14308
Source
Escherichia Coli.
Synonyms
Protein Kinase AMP-Activated Gamma 1 Non-Catalytic Subunit, 5'-AMP-Activated Protein Kinase Subunit Gamma-1, AMPK Gamma1 Chain, AMPKg.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PRKAG1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 354 amino acids (1-331) and having a molecular mass of 40.0kDa.
PRKAG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PRKAG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
The AMP-activated protein kinase (AMPK) is regulated by a subunit called PRKAG1. AMPK, a heterotrimer, consists of a catalytic alpha subunit and non-catalytic beta and gamma subunits. Playing a crucial role in cellular energy regulation, AMPK acts as an energy sensor. PRKAG1 is identified as one of the gamma regulatory subunits of AMPK. Notably, there are alternative splice transcript variants that encode different isoforms of this protein.
Description
Recombinantly produced in E.coli, PRKAG1 Human is a single, non-glycosylated polypeptide chain. It comprises 354 amino acids (1-331) and exhibits a molecular weight of 40.0kDa. For purification using proprietary chromatographic techniques, PRKAG1 is fused to a 23 amino acid His-tag at its N-terminus.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The PRKAG1 solution is prepared at a concentration of 0.25mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. It's important to avoid repeated freezing and thawing of the product.
Purity
SDS-PAGE analysis confirms that the purity of the product is greater than 85.0%.
Synonyms
Protein Kinase AMP-Activated Gamma 1 Non-Catalytic Subunit, 5'-AMP-Activated Protein Kinase Subunit Gamma-1, AMPK Gamma1 Chain, AMPKg.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMETVISS DSSPAVENEH PQETPESNNS VYTSFMKSHR CYDLIPTSSK LVVFDTSLQV KKAFFALVTN GVRAAPLWDS KKQSFVGMLT ITDFINILHR YYKSALVQIY ELEEHKIETW REVYLQDSFK PLVCISPNAS LFDAVSSLIR NKIHRLPVID PESGNTLYIL THKRILKFLK LFITEFPKPE FMSKSLEELQ IGTYANIAMV RTTTPVYVAL GIFVQHRVSA LPVVDEKGRV VDIYSKFDVI NLAAEKTYNN LDVSVTKALQ HRSHYFEGVL KCYLHETLET IINRLVEAEV HRLVVVDEND VVKGIVSLSD ILQALVLTGG EKKP
Product Science Overview
Structure and Function
AMPK acts as an energy sensor in cells, monitoring the cellular energy status. When cellular energy levels are low, AMPK is activated to restore energy balance by switching on catabolic pathways that generate ATP and switching off anabolic pathways that consume ATP . The gamma subunit, PRKAG1, is essential for the regulation of AMPK activity. It binds to AMP and ATP, which are crucial for the enzyme’s activation and regulation .
Recombinant Human PRKAG1
Biological Importance
AMPK is involved in several critical cellular processes, including:
- Regulation of Metabolism: AMPK regulates the activities of key metabolic enzymes through phosphorylation, ensuring cellular energy homeostasis .
- Response to Cellular Stress: It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways .
- Role in Diseases: Dysregulation of AMPK activity is associated with various diseases, including metabolic disorders, cancer, and cardiovascular diseases .
Applications in Research
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