PRKACB Human

Protein Kinase CAMP-Dependent Catalytic Beta Human Recombinant
Cat. No.
BT14219
Source
Escherichia Coli.
Synonyms
Protein Kinase CAMP-Dependent Catalytic Beta, PKA C-Beta, EC 2.7.11.11, PKACB, CAMP-Dependent Protein Kinase Catalytic Beta Subunit Isoform 4ab, CAMP-Dependent Protein Kinase Catalytic Subunit Beta, Protein Kinase A Catalytic Subunit Beta, EC 2.7.11, PRKACB.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PRKACB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 421 amino acids (1-398) and having a molecular mass of 48.6kDa.
PRKACB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Protein Kinase CAMP-Dependent Catalytic Beta (PRKACB), a member of the Ser/Thr protein kinase family, functions as a catalytic subunit of cAMP-dependent protein kinase. This kinase plays a crucial role in mediating cAMP signaling, which is essential for various cellular processes. Upon activation by cAMP, PRKACB phosphorylates target proteins, transmitting signals initiated by receptor binding to GPCRs. Its involvement spans cell proliferation, cell cycle regulation, differentiation, microtubule dynamics, chromatin structure modulation, nuclear envelope dynamics, intracellular transport, and ion flux.
Description
Recombinant PRKACB, expressed in E.coli, is a non-glycosylated polypeptide chain consisting of 421 amino acids (residues 1-398). With a molecular weight of 48.6 kDa, this protein includes a 23 amino acid His-tag at its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The PRKACB solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of PRKACB is determined to be greater than 80% using SDS-PAGE analysis.
Synonyms
Protein Kinase CAMP-Dependent Catalytic Beta, PKA C-Beta, EC 2.7.11.11, PKACB, CAMP-Dependent Protein Kinase Catalytic Beta Subunit Isoform 4ab, CAMP-Dependent Protein Kinase Catalytic Subunit Beta, Protein Kinase A Catalytic Subunit Beta, EC 2.7.11, PRKACB.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAYREP PCNQYTGTTT ALQKLEGFAS RLFHRHSKGT AHDQKTALEN DSLHFSEHTA LWDRSMKEFL AKAKEDFLKK WENPTQNNAG LEDFERKKTL GTGSFGRVML VKHKATEQYY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVRLEYA FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDHQGY IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEDIRVSI TEKCAKEFGE F.

Product Science Overview

Gene and Protein Structure

The PRKACB gene is located on chromosome 1 at the band 1p31.1 and spans approximately 160,000 base pairs . The gene produces multiple transcript variants through alternative splicing, resulting in different isoforms of the protein . The protein itself is a catalytic subunit of the cAMP-dependent protein kinase (PKA), which is a tetramer composed of two regulatory and two catalytic subunits .

Function and Mechanism

The primary function of PRKACB is to mediate the effects of cAMP within the cell. cAMP is a signaling molecule that activates PKA by binding to its regulatory subunits, causing the release of the active catalytic subunits . These catalytic subunits then phosphorylate various target proteins, leading to changes in their activity, localization, or interaction with other proteins .

Biological Significance

PRKACB is involved in numerous cellular processes, including:

  • Regulation of Metabolism: By phosphorylating enzymes involved in metabolic pathways, PRKACB helps regulate glucose and lipid metabolism .
  • Cell Growth and Differentiation: PRKACB plays a role in cell cycle regulation and differentiation, influencing cell growth and development .
  • Memory and Learning: In the nervous system, PRKACB is involved in synaptic plasticity, which is essential for memory formation and learning .
Clinical Relevance

Mutations or dysregulation of PRKACB can lead to various diseases. For instance, alterations in cAMP signaling pathways have been implicated in cancer, cardiovascular diseases, and neurological disorders . Understanding the role of PRKACB in these pathways can provide insights into potential therapeutic targets for these conditions .

Research and Applications

Recombinant human PRKACB is widely used in research to study its function and regulation. It is also used in drug discovery to screen for compounds that can modulate its activity . The availability of recombinant PRKACB allows for detailed biochemical and structural studies, which are essential for developing targeted therapies .

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