PRKACA Human, sf9
Sf9, Baculovirus cells.
cAMP-dependent protein kinase alpha-catalytic subunit, EC 2.7.11.11, PKA C-alpha, PKACA, PRKACA, MGC48865, MGC102831.
Greater than 85% as determined by SDS-PAGE.
Description
PRKACA Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 578 amino acids (1-351 a.a.) and having a molecular mass of 67kDa (Migrates at 50-70kDa on SDS-PAGE under reducing conditions). PRKACA is expressed with a 227 amino acid GST Tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
cAMP-dependent protein kinase alpha-catalytic subunit, EC 2.7.11.11, PKA C-alpha, PKACA, PRKACA, MGC48865, MGC102831.
Sf9, Baculovirus cells.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMGN AAAAKKGSEQ ESVKEFLAKA KEDFLKKWES PAQNTAHLDQ FERIKTLGTG SFGRVMLVKH KETGNHYAMK ILDKQKVVKL KQIEHTLNEK RILQAVNFPF LVKLEFSFKD NSNLYMVMEY VPGGEMFSHL RRIGRFSEPH ARFYAAQIVL TFEYLHSLDL IYRDLKPENL LIDQQGYIQV TDFGFAKRVK GRTWTLCGTP EYLAPEIILS KGYNKAVDWW ALGVLIYEMA AGYPPFFADQ PIQIYEKIVS GKVRFPSHFS SDLKDLLRNL LQVDLTKRFG NLKNGVNDIK NHKWFATTDW IAIYQRKVEA PFIPKFKGPG DTSNFDDYEE EEIRVSINEK CGKEFSEF.
Product Science Overview
The cAMP-dependent protein kinase A (PKA) catalytic subunit alpha, also known as PRKACA, is a crucial enzyme in various cellular processes. This enzyme is a part of the PKA family, which plays a significant role in the regulation of metabolism, gene expression, and cell cycle progression. The human recombinant form of this enzyme, expressed in Sf9 insect cells, is widely used in research and therapeutic applications.
PKA is a serine/threonine kinase that exists as a tetrameric holoenzyme composed of two regulatory ® and two catalytic © subunits in its inactive form. The binding of cyclic AMP (cAMP) to the regulatory subunits causes the dissociation of the holoenzyme, releasing the active catalytic subunits . The catalytic subunit alpha (PRKACA) is one of the three catalytic subunits identified in humans .
The activation of PKA is tightly regulated by the intracellular levels of cAMP. When cAMP levels rise, it binds to the regulatory subunits, causing a conformational change that releases the catalytic subunits . These free catalytic subunits then phosphorylate various target proteins, leading to changes in their activity, localization, or interaction with other proteins .
PKA-mediated phosphorylation is involved in numerous cellular processes, including:
- Metabolism: PKA regulates glycogen, sugar, and lipid metabolism.
- Gene Expression: PKA influences the transcription of various genes by phosphorylating transcription factors.
- Cell Cycle: PKA plays a role in cell cycle progression and differentiation.
- Apoptosis: PKA can either promote or inhibit apoptosis depending on the cellular context .
Mutations or dysregulation of PRKACA have been associated with several diseases, including:
- Cushing’s Syndrome: Constitutive activation of PRKACA due to somatic mutations or genomic duplications can lead to hyperplasias and adenomas of the adrenal cortex .
- Cancer: PRKACA has been implicated in breast cancer resistance to HER2-targeted therapies and in the neoplastic behavior of neuroendocrine cancers .
