PRKAB1 Human

Protein Kinase, AMP-Activated, Beta 1 non-Catalytic Subunit Human Recombinant
Cat. No.
BT14076
Source
E.coli.
Synonyms
AMPK, HAMPKb, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb,PRKAB1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PRKAB1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 293 amino acids (1-270 a.a.) and having a molecular mass of 32.8 kDa. The PRKAB1 is fused to a 23 amino acid His Tag at N-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
5'-AMP-activated protein kinase subunit beta-1 (PRKAB1) is involved in inhibiting the production of proteins, carbohydrates, and lipids, as well as regulating cell growth and proliferation. AMPK, a heterotrimeric complex, consists of an alpha catalytic subunit alongside non-catalytic beta and gamma subunits. AMPK exerts its effects through directly phosphorylating metabolic enzymes and indirectly influencing gene expression by phosphorylating transcription regulators. PRKAB1 plays a role in controlling cell polarity by restructuring the actin cytoskeleton, possibly by activating myosin indirectly. The beta subunit, being non-catalytic, serves as a platform for assembling the AMPK complex. Its C-terminus facilitates this by binding to alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2, or PRKAG3).
Description
Recombinant human PRKAB1, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 293 amino acids (amino acids 1-270) with a molecular weight of 32.8 kDa. The PRKAB1 protein has a 23 amino acid His Tag fused to its N-terminus. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The PRKAB1 protein solution is provided at a concentration of 0.5 mg/mL in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For optimal storage, keep the vial at 4°C if the entire contents will be used within 2-4 weeks. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
AMPK, HAMPKb, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb,PRKAB1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGNTSSE RAALERHGGH KTPRRDSSGG TKDGDRPKIL MDSPEDADLF HSEEIKAPEK EEFLAWQHDL EVNDKAPAQA RPTVFRWTGG GKEVYLSGSF NNWSKLPLTR SHNNFVAILD LPEGEHQYKF FVDGQWTHDP SEPIVTSQLG TVNNIIQVKK TDFEVFDALM VDSQKCSDVS ELSSSPPGPY HQEPYVCKPE ERFRAPPILP PHLLQVILNK DTGISCDPAL LPEPNHVMLN HLYALSIKDG VMVLSATHRY KKKYVTTLLY KPI

Product Science Overview

Introduction

Protein Kinase, AMP-Activated, Beta 1 Non-Catalytic Subunit, also known as PRKAB1, is a regulatory subunit of the AMP-activated protein kinase (AMPK) complex. AMPK is a crucial energy-sensing enzyme that monitors cellular energy status and plays a significant role in maintaining energy homeostasis. The AMPK complex is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits .

Structure and Function

The PRKAB1 gene encodes the beta-1 subunit of the AMPK complex. This subunit is non-catalytic but is essential for the proper functioning of the AMPK enzyme. The AMPK complex is activated in response to cellular metabolic stresses, such as low glucose levels, hypoxia, and exercise. Upon activation, AMPK phosphorylates and inactivates key enzymes involved in regulating de novo biosynthesis of fatty acids and cholesterol, such as acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR) .

Role in Cellular Metabolism

AMPK acts as a metabolic master switch, regulating several intracellular systems, including the uptake of glucose, the oxidation of fatty acids, and the biogenesis of mitochondria. The beta-1 subunit, encoded by PRKAB1, is believed to be a positive regulator of AMPK activity. Myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK .

Genetic Information

The PRKAB1 gene is located on chromosome 12 in humans. It has several aliases, including AMPK subunit beta-1, AMPK beta 1, and 5’-AMP-activated protein kinase subunit beta-1 . The gene is expressed in various tissues, with high expression levels in the rectum, kidney, body of the stomach, spleen, renal medulla, upper lobe of the left lung, body of the pancreas, right lung, minor salivary glands, and left adrenal gland .

Clinical Significance

Mutations or dysregulation of the PRKAB1 gene have been associated with several diseases, including Krabbe disease and colorectal cancer . The AMPK pathway, in which PRKAB1 plays a crucial role, is a potential therapeutic target for metabolic disorders, cancer, and cardiovascular diseases.

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