PRKAA1 Human
Protein Kinase, AMP-Activated, Alpha 1 Human Recombinant
Cat. No.
BT13992
Source
E.coli.
Synonyms
5'-AMP-activated protein kinase catalytic subunit alpha-1, Protein Kinase, AMP-Activated, Alpha 1, PRKAA1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, AMPK1, 5-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1, AMPK, AMPKa11.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
PRKAA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 302 amino acids (1-279a.a) and having a molecular mass of 34.3kDa.
PRKAA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PRKAA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Protein Kinase, AMP-Activated, Alpha 1 (PRKAA1), encoded by the PRKAA1 gene, is the catalytic subunit of AMPK, an energy sensor protein kinase crucial for cellular energy metabolism regulation. This kinase directly phosphorylates various metabolic enzymes, adjusting their activity. Notably, AMPK safeguards cells from stresses leading to ATP depletion by deactivating ATP-dependent biosynthetic pathways.
Description
This product consists of recombinant human PRKAA1, a non-glycosylated polypeptide chain expressed in E. coli. Containing 302 amino acids (1-279a.a), it has a molecular weight of 34.3 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic methods.
Physical Appearance
A colorless solution that has been sterilized through filtration.
Formulation
The PRKAA1 solution is provided at a concentration of 1 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be kept at 4°C. For longer storage, freezing at -20°C is recommended. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Avoid repeated freezing and thawing cycles.
Purity
Purity exceeds 85% based on SDS-PAGE analysis.
Synonyms
5'-AMP-activated protein kinase catalytic subunit alpha-1, Protein Kinase, AMP-Activated, Alpha 1, PRKAA1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, AMPK1, 5-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1, AMPK, AMPKa11.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMRRLSSW RKMATAEKQK HDGRVKIGHY ILGDTLGVGT FGKVKVGKHE LTGHKVAVKI LNRQKIRSLD VVGKIRREIQ NLKLFRHPHI IKLYQVISTP SDIFMVMEYV SGGELFDYIC KNGRLDEKES RRLFQQILSG VDYCHRHMVV HRDLKPENVL LDAHMNAKIA DFGLSNMMSD GEFLRTSCGS PNYAAPEVIS GRLYAGPEVD IWSSGVILYA LLCGTLPFDD DHVPTLFKKI CDGIFYTPQY LNPSVISLLK HMLQVDPMKR ATIKDIREHE WF.
Product Science Overview
Structure and Function
AMPK is a heterotrimeric complex composed of a catalytic alpha subunit (PRKAA1), and regulatory beta and gamma subunits . The alpha subunit is responsible for the kinase activity, while the beta and gamma subunits regulate the enzyme’s activity and localization .
AMPK acts as a cellular energy sensor, activated by an increase in the AMP/ATP ratio, which occurs during metabolic stress . Once activated, AMPK phosphorylates various downstream targets to restore energy balance by:
Biological Significance
AMPK plays a pivotal role in maintaining cellular energy homeostasis and is conserved across all eukaryotic cells . It protects cells from ATP depletion by switching off ATP-consuming biosynthetic pathways . This makes AMPK a key regulator in various physiological processes, including:
- Metabolic regulation: AMPK modulates the activity of key metabolic enzymes, influencing glucose and lipid metabolism .
- Cell growth and proliferation: By regulating mTOR signaling, AMPK controls cell growth and proliferation .
- Autophagy: AMPK activation promotes autophagy, a process that degrades and recycles cellular components .
Clinical Relevance
Mutations or dysregulation of AMPKα1 have been associated with several diseases, including:
- Metabolic disorders: Impaired AMPK signaling is linked to obesity, type 2 diabetes, and metabolic syndrome .
- Cancer: AMPK acts as a tumor suppressor by inhibiting cell growth and proliferation. Dysregulation of AMPK signaling can contribute to cancer progression .
- Cardiovascular diseases: AMPK plays a protective role in the heart by enhancing energy production and reducing oxidative stress .
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