PPP1CA Antibody

Protein Phosphatase 1 Catalytic Subunit Alpha, Mouse Anti Human
Cat. No.
BT24075
Source
Synonyms
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, protein phosphatase 1 catalytic subunit alpha isozyme, MGC1674, MGC15877, PP1alpha.
Appearance
Sterile Filtered clear solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
PPP1CA, a catalytic subunit of protein phosphatase 1 (PP1), is a serine/threonine-specific phosphatase. PP1 regulates various cellular processes, including cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity is observed in end-stage heart failure. Research using human and mice models suggests PP1 significantly regulates cardiac function. Studies in mice also propose a role for PP1 as a suppressor of learning and memory.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
1 mg/ml in PBS, pH 7.4, with 0.1% sodium azide.
Storage Procedures
Store at 4°C for up to 1 month. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
The PPP1CA antibody has been validated for specificity and reactivity by ELISA and Western blot analysis. Optimal working dilutions should be determined for each application. The recommended dilution range for Western blot analysis is 1:500 to 1:2,000, with a starting dilution of 1:1,000.
Synonyms
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, protein phosphatase 1 catalytic subunit alpha isozyme, MGC1674, MGC15877, PP1alpha.
Purification Method
PPP1CA antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P4G3AT.
Immunogen
Anti-human PPP1CA mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPP1CA amino acids 30-299 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.

Product Science Overview

Overview

Protein Phosphatase 1 Catalytic Subunit Alpha (PPP1CA) is a critical enzyme involved in various cellular processes. It is one of the three catalytic subunits of Protein Phosphatase 1 (PP1), a serine/threonine-specific protein phosphatase . PP1 is known for its role in dephosphorylating a wide range of biological targets, thereby regulating their activity.

Gene and Protein Structure

The PPP1CA gene encodes the alpha subunit of the PP1 complex. This gene is broadly expressed and associates with over 200 regulatory proteins to form holoenzymes . These holoenzymes dephosphorylate their targets with high specificity, which is crucial for maintaining cellular functions .

Biological Functions

PPP1CA is involved in several key cellular processes, including:

  • Cell Division: PP1 plays a role in the regulation of the cell cycle by dephosphorylating specific substrates involved in cell division .
  • Glycogen Metabolism: PP1 is essential for glycogen synthesis and breakdown, thereby regulating energy storage and release .
  • Muscle Contractility: PP1 regulates muscle contraction by dephosphorylating myosin light chains .
  • Protein Synthesis: PP1 is involved in the regulation of protein synthesis by dephosphorylating translation factors .
  • HIV-1 Viral Transcription: PP1 has been implicated in the regulation of HIV-1 transcription .
Clinical Significance

Increased PP1 activity has been observed in the end stage of heart failure, suggesting its role in cardiac function regulation . Deregulation of PP1 is also implicated in diabetes and multiple types of cancer . Understanding the function and regulation of PPP1CA is therefore critical for developing therapeutic strategies for these diseases.

Mouse Anti-Human PPP1CA Antibody

Mouse anti-human PPP1CA antibodies are used in research to study the expression and function of PPP1CA in various biological contexts. These antibodies are typically generated by immunizing mice with human PPP1CA protein, leading to the production of specific antibodies that can be harvested and used in experiments.

Research Applications

Mouse anti-human PPP1CA antibodies are used in various research applications, including:

  • Western Blotting: To detect PPP1CA protein levels in different samples.
  • Immunoprecipitation: To isolate PPP1CA and its interacting partners.
  • Immunofluorescence: To visualize the localization of PPP1CA within cells.
  • Enzyme-Linked Immunosorbent Assay (ELISA): To quantify PPP1CA protein levels in biological samples.

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