Introduction
PPME1 is an enzyme that acts as a protein phosphatase methylesterase. It specifically targets and demethylates protein phosphatase 2A (PP2A), a crucial enzyme involved in regulating cell growth and cycle progression. By removing a methyl group from PP2A, PPME1 inactivates it. This inactivation has significant implications for cellular processes, as PP2A typically acts as a growth inhibitor and promotes cell cycle arrest. PPME1's activity can be countered by okadaic acid, a known inhibitor of protein phosphatases. The enzyme is highly conserved across various species, highlighting its fundamental role in cellular regulation. Structurally, PPME1 possesses a distinct motif commonly found in lipases, enzymes responsible for breaking down fats. This motif includes a catalytic triad with an activated serine residue that serves as the primary nucleophile during enzymatic reactions.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
A solution containing 1mg of PPME1 Antibody per milliliter. It is prepared in a buffer solution of phosphate-buffered saline (PBS) at a pH of 7.4. The solution also contains 10% glycerol as a stabilizing agent and 0.02% sodium azide as a preservative.
Storage Procedures
For short-term storage of up to one month, keep the antibody solution refrigerated at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To maintain optimal antibody activity, avoid repeated cycles of freezing and thawing.
Stability / Shelf Life
The antibody remains stable for a period of 12 months when stored at -20°C. At a refrigerated temperature of 4°C, the antibody can be stored for up to 1 month.
Applications
The PPME1 antibody has been rigorously tested and validated for use in ELISA and Western blot analyses. These tests confirm its specificity and reactivity with the target protein. However, it's essential to note that optimal antibody concentrations may vary depending on the specific application and experimental conditions. Therefore, researchers are advised to perform titration experiments to determine the most effective antibody concentration for their particular needs.
Synonyms
Protein phosphatase methylesterase 1, PME-1, FLJ22226, EC 3.1.1.
Purification Method
PPME1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human PPME1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPME1 amino acids 1-386 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and K light chain.
