PPIF Antibody
Description
Product Specs
Product Science Overview
Cyclophilin-F, also known as peptidylprolyl isomerase F (PPIF), is a mitochondrial matrix protein that plays a crucial role in various cellular processes. It is a member of the cyclophilin family, which is characterized by their peptidyl-prolyl cis-trans isomerase (PPIase) activity. Cyclophilin-F is involved in protein folding, mitochondrial permeability transition pore (mPTP) regulation, and apoptosis.
Cyclophilin-F is a 22 kDa protein that is encoded by the PPIF gene. It is primarily localized in the mitochondrial matrix, where it interacts with other mitochondrial proteins to regulate the mPTP. The mPTP is a multi-protein complex that controls the permeability of the mitochondrial membrane, and its opening can lead to cell death through apoptosis or necrosis.
Cyclophilin-F’s PPIase activity is essential for its role in protein folding and function. It catalyzes the cis-trans isomerization of proline residues in polypeptide chains, which is a critical step in the proper folding of proteins. Additionally, Cyclophilin-F is known to bind to cyclosporin A, a potent immunosuppressive drug, which inhibits its PPIase activity and prevents mPTP opening.
The mouse anti-human Cyclophilin-F antibody is a monoclonal antibody that is specifically designed to detect human Cyclophilin-F. Monoclonal antibodies are produced by identical immune cells that are clones of a unique parent cell, ensuring high specificity and consistency in their binding to the target antigen.
Production and Purification
The mouse anti-human Cyclophilin-F antibody is produced by immunizing mice with a recombinant human Cyclophilin-F protein. The antibody-producing B cells are then isolated and fused with myeloma cells to create hybridomas, which are capable of continuous growth and antibody production. The antibodies are subsequently purified using protein-G affinity chromatography to ensure high purity and specificity.
Applications
The mouse anti-human Cyclophilin-F antibody is widely used in various research applications, including:
- Western Blotting (WB): To detect and quantify Cyclophilin-F protein levels in different samples.
- Immunohistochemistry (IHC): To visualize the localization and distribution of Cyclophilin-F in tissue sections.
- Enzyme-Linked Immunosorbent Assay (ELISA): To measure Cyclophilin-F concentrations in biological fluids.
- Flow Cytometry: To analyze the expression of Cyclophilin-F on the surface of cells.
Storage and Handling
The antibody is typically supplied in a liquid form, with a concentration of 1 mg/ml. It should be stored at 4ºC for short-term use (1-2 weeks) and at -20ºC or below for long-term storage. It is important to avoid multiple freeze-thaw cycles to maintain the antibody’s stability and functionality.
