PPARGC1A Antibody
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC-1-alpha, PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha, Ligand effect modulator 6, LEM6, PGC1, PGC1A, PPARGC1, PPARGC1A.
Description
Product Specs
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC-1-alpha, PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha, Ligand effect modulator 6, LEM6, PGC1, PGC1A, PPARGC1, PPARGC1A.
PPARGC1A antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT25C8AT.
Anti-human PPARGC1A mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPARGC1A protein 300-540 amino acids purified from E. coli.
Mouse IgG2a heavy chain and k light chain.
Product Science Overview
PGC-1α is known as the master regulator of mitochondrial biogenesis. It interacts with the nuclear receptor PPAR-γ (Peroxisome Proliferator-Activated Receptor Gamma), which allows it to interact with multiple transcription factors . This interaction is essential for the regulation of genes involved in energy metabolism, making PGC-1α a key player in processes such as:
- Mitochondrial biogenesis: The creation of new mitochondria within cells .
- Gluconeogenesis: The production of glucose from non-carbohydrate sources .
- Muscle fiber type determination: Influencing the development of slow-twitch muscle fibers, which are more efficient for endurance activities .
PGC-1α is also involved in controlling blood pressure, regulating cellular cholesterol homeostasis, and the development of obesity .
PGC-1α is activated by various external physiological stimuli, including:
- Reactive oxygen species (ROS) and reactive nitrogen species (RNS): These are by-products of metabolism that are upregulated during cellular stress .
- Fasting: Increases gluconeogenic gene expression, including hepatic PGC-1α .
- Cold exposure: Links environmental stimuli to adaptive thermogenesis .
- Endurance exercise: Activates the PGC-1α gene in human skeletal muscle, increasing autophagy and the unfolded protein response .
The Mouse Anti-Human PPARG Coactivator 1 Alpha antibody is a monoclonal antibody used in research to detect and study the PGC-1α protein. This antibody is crucial for experiments involving:
- Western blotting: To detect PGC-1α protein levels in various samples.
- Immunohistochemistry: To visualize the localization of PGC-1α within tissues.
- Flow cytometry: To analyze the expression of PGC-1α in different cell populations.
By using this antibody, researchers can gain insights into the role of PGC-1α in energy metabolism, mitochondrial biogenesis, and other related processes.
