PON2 Human

PON2 is an intracellular enzyme, unlike PON1 and PON3, which are secreted extracellularly . It has a significant role in protecting cells from oxidative stress and is involved in various physiological processes. PON2 exhibits lactonase activity, hydrolyzing lactones such as N-(3-oxododecanoyl)-L-homoserine lactone (3OC12-HSL), a quorum sensing molecule . This activity is crucial for its role in modulating bacterial infections and biofilm formation .

PON2 is associated with several diseases, including cancer, cardiovascular diseases, neurodegeneration, and diabetes . Its ability to reduce oxidative stress makes it a vital enzyme in preventing the progression of these diseases. Clinical studies have highlighted its significance as a biomarker for various conditions .

Recombinant PON2 (rPON2) has been engineered to study its structure and function in detail. The recombinant version is often produced in E. coli and refolded from inclusion bodies to obtain an active enzyme . This allows researchers to investigate the enzyme’s catalytic properties and its interactions with other molecules. For instance, rPON2 has been shown to inhibit the biofilm formation of Pseudomonas aeruginosa more effectively than PON1 .

PON2 undergoes several post-translational modifications, including glycosylation and ubiquitination . These modifications can influence its catalytic activity and stability. Understanding these modifications is crucial for developing therapeutic applications of PON2.