PON1 Human, HEK

Paraoxonase-1 is produced in the liver and distributed through the bloodstream, where it interacts with HDL. The recombinant form of PON1, produced in Human Embryonic Kidney (HEK) cells, is a single, glycosylated polypeptide chain consisting of 346 amino acids with a molecular mass of approximately 39.0 kDa . This recombinant enzyme is fused to a 6-amino acid His-tag at the C-terminus and is purified using proprietary chromatographic techniques .

PON1 is known for its ability to hydrolyze a wide range of substrates, including cyclic carbonates, lactones, and nerve gases . It requires activation by PPAR-gamma, which leads to its synthesis and release from liver tissue, ultimately contributing to the reduction of atherosclerosis . The enzyme’s anti-atherosclerotic properties are attributed to its role in metabolizing inflammatory lipid peroxides .

The specific activity of PON1 is defined by its ability to hydrolyze p-nitrophenyl acetate to p-nitrophenol at a rate of more than 2,500 pmol/min/µg at pH 7.5 and 37°C . This high level of activity underscores its efficiency in detoxifying harmful compounds and protecting against oxidative stress.

Recombinant PON1 is used extensively in laboratory research, particularly in studies related to cardiovascular diseases and toxicology . It is supplied as a sterile, filtered, colorless solution containing 20% glycerol and phosphate-buffered saline (pH 7.4) . For optimal stability, it should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods . To prevent degradation, it is recommended to avoid multiple freeze-thaw cycles and to add a carrier protein for long-term storage .