POLR2J3 Human

The POLR2J3 gene produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to the DNA-directed RNA polymerase II polypeptide J . The human recombinant form of POLR2J3 is typically produced in Escherichia coli (E. coli) and is a single polypeptide chain containing 138 amino acids, with a molecular mass of approximately 15.5 kDa . This recombinant protein is often fused to a 23 amino acid His-tag at the N-terminus to facilitate purification .

POLR2J3 is a component of the RNA polymerase II complex, which synthesizes messenger RNA (mRNA) precursors and many functional non-coding RNAs. RNA polymerase II is the central component of the basal transcription machinery in eukaryotic cells. It is composed of mobile elements that move relative to each other, with RPB11 (another name for POLR2J3) being part of the core element with the central large cleft .

The transcription of DNA into RNA by RNA polymerase II is a critical step in gene expression, influencing various cellular processes and functions. POLR2J3, as a subunit of this complex, plays a role in ensuring the proper transcription of genetic information. Disruptions or mutations in the POLR2J3 gene can potentially lead to various diseases, although specific associations are still being studied .

Recombinant POLR2J3 is widely used in research to study the mechanisms of transcription and the role of RNA polymerase II in gene expression. The availability of human recombinant POLR2J3 allows researchers to conduct detailed biochemical and structural analyses, contributing to our understanding of transcriptional regulation and its implications in health and disease .