POLR2H Human

Polymerase (RNA) II (DNA directed) Polypeptide H Human Recombinant
Cat. No.
BT4780
Source
Escherichia Coli.
Synonyms
DNA-directed RNA polymerases I, II, and III subunit RPABC3, RNA polymerases I, II, and III subunit ABC3, DNA-directed RNA polymerase II subunit H, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide, RPB17, RPB8 homolog, hRPB8, POLR2H, polymerase (RNA) II (DNA directed) polypeptide H, RPB8, RPABC3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

POLR2H Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids (1-150 a.a) and having a molecular mass of 19.5kDa.
POLR2H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
POLR2H is a crucial and highly conserved subunit found in RNA polymerase II. This subunit is also present in RNA polymerases I and III, the other two eukaryotic DNA-directed RNA polymerases. These three eukaryotic RNA polymerases are intricate multisubunit enzymes that play a critical role in transcribing nuclear genes. POLR2H is responsible for encoding this essential subunit.
Description
Recombinant POLR2H, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 173 amino acids (specifically, amino acids 1 to 150). It has a molecular weight of 19.5 kDa. This protein is fused with a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for sterility.
Formulation
The POLR2H protein solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the POLR2H protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
DNA-directed RNA polymerases I, II, and III subunit RPABC3, RNA polymerases I, II, and III subunit ABC3, DNA-directed RNA polymerase II subunit H, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide, RPB17, RPB8 homolog, hRPB8, POLR2H, polymerase (RNA) II (DNA directed) polypeptide H, RPB8, RPABC3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGILFE DIFDVKDIDP EGKKFDRVSR LHCESESFKM DLILDVNIQI YPVDLGDKFR LVIASTLYED GTLDDGEYNP TDDRPSRADQ FEYVMYGKVY RIEGDETSTE AATRLSAYVS YGGLLMRLQG DANNLHGFEV DSRVYLLMKK LAF.

Product Science Overview

Introduction

Polymerase (RNA) II (DNA directed) Polypeptide H, also known as POLR2H, is a crucial component of the RNA polymerase II complex in humans. This enzyme is responsible for transcribing DNA into RNA, a fundamental process in gene expression. The human recombinant form of this polypeptide is often used in research to study its function and role in transcription.

Structure and Function

POLR2H is a highly conserved subunit shared by RNA polymerases I, II, and III. These polymerases are essential for the transcription of nuclear genes in eukaryotic cells. RNA polymerase II, in particular, is responsible for synthesizing messenger RNA (mRNA) precursors and most small nuclear RNA (snRNA) and microRNA .

The POLR2H subunit plays a critical role in the assembly and stability of the RNA polymerase II complex. It interacts with other subunits to form a functional enzyme capable of accurately transcribing DNA into RNA. This process involves the use of ribonucleoside triphosphates as substrates .

Genetic Information

The POLR2H gene is located on chromosome 3q27.1 and is highly conserved across different species, including humans, mice, and rats . Alternative splicing of this gene results in multiple transcript variants, which may have different functions or regulatory roles .

Biological Significance

RNA polymerase II is a multiprotein complex that transcribes DNA into RNA, which is then processed into mRNA. This mRNA serves as a template for protein synthesis, making RNA polymerase II essential for gene expression and cellular function . The POLR2H subunit is integral to this process, ensuring the proper assembly and function of the polymerase complex.

Applications in Research

The human recombinant form of POLR2H is widely used in research to study its role in transcription and its interactions with other subunits. By using recombinant proteins, researchers can investigate the molecular mechanisms underlying gene expression and identify potential targets for therapeutic intervention.

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