POLR1C Human

POLR1C is a protein-coding gene that encodes a polypeptide chain consisting of 369 amino acids, with a molecular mass of approximately 41.6 kDa . This polypeptide is a part of the catalytic core of RNA polymerase I (Pol I) and RNA polymerase III (Pol III), which are DNA-dependent RNA polymerases. These enzymes synthesize ribosomal RNA (rRNA) precursors and short non-coding RNAs, including 5S rRNA, small nuclear RNAs (snRNAs), transfer RNAs (tRNAs), and microRNAs (miRNAs) .

The primary role of POLR1C within the RNA polymerase I and III complexes is to facilitate the transcription of ribosomal RNA (rRNA) genes and other small RNA molecules. This transcription process is essential for ribosome biogenesis and protein synthesis, which are critical for cell growth and proliferation .

Mutations or defects in the POLR1C gene have been associated with Treacher Collins syndrome, a genetic disorder characterized by craniofacial deformities. This highlights the importance of POLR1C in normal cellular function and development .

Recombinant Human POLR1C is produced in E. coli and is typically fused to a 23 amino acid His-tag at the N-terminus to facilitate purification. The recombinant protein is purified using proprietary chromatographic techniques to achieve a purity greater than 80% as determined by SDS-PAGE . The protein solution is formulated with 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT for stability and storage .

For optimal stability, the recombinant POLR1C protein should be stored at 4°C if used within 2-4 weeks. For longer-term storage, it is recommended to freeze the protein at -20°C, with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain protein integrity .