POLE3 Human

POLE3 is a human recombinant protein produced in Escherichia coli (E. coli). It is a single polypeptide chain consisting of 170 amino acids, with a molecular mass of approximately 19 kDa . The recombinant version of POLE3 is often fused to a 23 amino acid His-tag at the N-terminus to facilitate purification through chromatographic techniques .

The primary function of POLE3 is to participate in the DNA replication process. It is a part of the DNA polymerase epsilon complex, which is responsible for synthesizing the leading strand during DNA replication. This complex ensures high-fidelity DNA synthesis, thereby preventing mutations and maintaining genomic stability.

The recombinant POLE3 is expressed in E. coli and purified to a high degree of purity, typically greater than 90% as determined by SDS-PAGE . The protein is formulated in a solution containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol, and 1mM DTT . This formulation helps maintain the stability and activity of the protein.

For short-term storage, POLE3 can be kept at 4°C if it will be used within 2-4 weeks. For longer-term storage, it is recommended to store the protein at -20°C. To prevent degradation, it is advisable to add a carrier protein such as 0.1% HSA or BSA and avoid multiple freeze-thaw cycles .

POLE3 is used in various research applications, including studies on DNA replication, repair mechanisms, and chromatin accessibility. Its role in maintaining genomic stability makes it a valuable tool for understanding the molecular mechanisms underlying these processes.