POLD4 Human

Polymerase Delta 4 Human Recombinant
Cat. No.
BT8272
Source
Escherichia Coli.
Synonyms
DNA polymerase delta subunit 4, p12, POLDS, DNA polymerase delta subunit p12, POLD4, Polymerase Delta 4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

POLD4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 130 amino acids (1-107) and having a molecular mass of 14.8kDa.
POLD4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Polymerase Delta 4, also called POLD4, is the smallest subunit of DNA polymerase delta. DNA polymerase delta plays a crucial role in DNA replication and repair, possessing both polymerase and 3' to 5' exonuclease activity. POLD4 enhances the activity of DNA polymerase delta and participates in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Additionally, POLD4 contributes to the PCNA-dependent activity of DNA polymerase delta.
Description
Recombinant human POLD4, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 130 amino acids (residues 1-107) with a molecular weight of 14.8 kDa. It features a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, filtered solution that is colorless.
Formulation
The POLD4 solution is provided at a concentration of 0.25 mg/ml and is formulated in 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 40% glycerol, and 2 mM DTT.
Stability
For short-term storage (2-4 weeks), store the solution at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To preserve protein integrity during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
The purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
DNA polymerase delta subunit 4, p12, POLDS, DNA polymerase delta subunit p12, POLD4, Polymerase Delta 4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGRKRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL.

Product Science Overview

Structure and Function

Pol δ is involved in the synthesis of the lagging strand during DNA replication. It also participates in various DNA repair mechanisms, including base excision repair (BER) and mismatch repair (MMR) . The enzyme’s activity is regulated by its interaction with proliferating cell nuclear antigen (PCNA), which acts as a sliding clamp to increase the processivity of DNA synthesis .

Recombinant Production

The production of recombinant human Pol δ has been achieved using various expression systems. One notable method involves the use of the Bombyx mori (silkworm) bioreactor, which allows for the large-scale production of the enzyme with high activity and purity . This system utilizes recombinant baculoviruses to infect silkworm larvae, resulting in the expression of the Pol δ complex. The recombinant enzyme produced in this manner has been shown to exhibit similar biochemical properties to the native form .

Research and Applications

Recombinant Pol δ is a valuable tool for studying the enzyme’s biochemical properties, regulation, and role in maintaining genomic stability. It has been used in various in vitro assays to investigate its function in DNA replication and repair . Understanding the mechanisms of Pol δ can provide insights into the etiology of human diseases, such as cancer, that result from genomic instability .

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THE BIOTEK
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