POGLUT1 Human
Protein O-Glucosyltransferase 1 Human Recombinant
Cat. No.
BT12278
Source
Sf9,
Baculovirus cells.
Synonyms
POGLUT1, C3orf9, CLP46, hCLP46, KDELCL1, KTELC1, Protein
O-glucosyltransferase 1, CAP10-like 46 kDa protein, KTEL motif-containing
protein 1, Myelodysplastic syndromes relative protein, O-glucosyltransferase
Rumi homolog, hRumi, Protein O-xylosyltransferase, MDSRP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
POGLUT1 Human Recombinant produced in in Sf9 Baculovirus
cells is a single, non-glycosylated polypeptide chain containing 377 amino
acids (24-392a.a) and having a molecular mass of 44.5kDa (Migrates at 40-57kDa
on SDS-PAGE under reducing conditions).
POGLUT1 is fused to a 8 amino acid His-tag at C-Terminus
and purified by proprietary chromatographic techniques.
Product Specs
Introduction
POGLUT1, a homologue of Rumi from Drosophila, is an endoplasmic reticulum (ER)-retaining glucosyltransferase. This enzyme catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to EGF repeats containing the consensus sequence C-X-S-X-P-C. POGLUT1 demonstrates a positive regulatory effect on Notch signaling pathways without impacting Notch ligand binding.
Description
Recombinant human POGLUT1, expressed in Sf9 Baculovirus cells, is a non-glycosylated polypeptide chain. This single chain protein consists of 377 amino acids (24-392a.a) and exhibits a molecular weight of 44.5kDa. Under reducing conditions on SDS-PAGE, it migrates between 40-57kDa. The protein is fused with an 8 amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The POGLUT1 solution is provided at a concentration of 0.25mg/ml in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
POGLUT1, C3orf9, CLP46, hCLP46, KDELCL1, KTELC1, Protein
O-glucosyltransferase 1, CAP10-like 46 kDa protein, KTEL motif-containing
protein 1, Myelodysplastic syndromes relative protein, O-glucosyltransferase
Rumi homolog, hRumi, Protein O-xylosyltransferase, MDSRP.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
RQKESGSKWK
VFIDQINRSL ENYEPCSSQN CSCYHGVIEE DLTPFRGGIS RKMMAEVVRR KLGTHYQITK NRLYRENDCM
FPSRCSGVEH FILEVIGRLP DMEMVINVRD YPQVPKWMEP AIPVFSFSKT SEYHDIMYPA WTFWEGGPAV
WPIYPTGLGR WDLFREDLVR SAAQWPWKKK NSTAYFRGSR TSPERDPLIL LSRKNPKLVD AEYTKNQAWK
SMKDTLGKPA AKDVHLVDHC KYKYLFNFRG VAASFRFKHL FLCGSLVFHV GDEWLEFFYP QLKPWVHYIP
VKTDLSNVQE LLQFVKANDD VAQEIAERGS QFIRNHLQMD DITCYWENLL SEYSKFLSYN VTRRKGYDQI
IPKMLKTELL EHHHHHH.
Product Science Overview
Structure and Function
POGLUT1 is an endoplasmic reticulum (ER)-retaining glucosyltransferase. The human recombinant form of POGLUT1 is typically produced in a Chinese Hamster Ovary (CHO) cell line and is characterized by a molecular mass of approximately 44 kDa . The enzyme’s activity is measured by its ability to hydrolyze UDP-Glucose, with a specific activity of more than 2 pmol/min/μg .
Biological Significance
The addition of glucose to serine residues by POGLUT1 is a critical step in the Notch signaling pathway, which is involved in various cellular processes, including differentiation, proliferation, and apoptosis . Dysregulation of this pathway can lead to several diseases, including cancer and congenital disorders.
Applications in Research
Storage and Stability
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