PNRC2 Human

Proline-Rich Nuclear Receptor Coactivator 2 Human Recombinant

Cat. No.

BT8060

Source

Escherichia Coli.

Synonyms

Proline-rich nuclear receptor coactivator 2, PNRC2.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

PNRC2 Human Recombinant produced in E. coli is a single polypeptide chain containing 162 amino acids (1-139) and having a molecular mass of 18kDa. PNRC2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Proline-Rich Nuclear Receptor Coactivator 2 (PNRC2) is a protein that helps regulate energy balance by influencing both energy storage and expenditure. It plays a role in nonsense-mediated mRNA decay (NMD), a process that degrades faulty messenger RNA molecules. PNRC2 acts as a link between the mRNA decapping complex and the NMD machinery, facilitating the breakdown of these aberrant mRNA molecules. It is essential for the localization of UPF1/RENT1, key components of the NMD pathway, to P-bodies, which are cellular structures involved in mRNA degradation. Additionally, PNRC2 functions as a nuclear receptor coactivator, influencing the activity of nuclear receptors involved in various cellular processes.

Description

This product consists of the human PNRC2 protein, recombinantly produced in E. coli bacteria. It is a single chain of 162 amino acids (specifically, amino acids 1 to 139 of the native protein sequence) with a molecular weight of 18kDa. For purification and detection purposes, the PNRC2 protein has a 23 amino acid His-tag attached to its N-terminus and is purified using proprietary chromatographic methods.

Physical Appearance

A clear solution that has been sterilized through filtration.

Formulation

The PNRC2 protein is supplied in a solution with a concentration of 1mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea, and 10% glycerol.

Stability

For short-term storage (up to 2-4 weeks), keep the product refrigerated at 4°C. For long-term storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for prolonged storage. To maintain product integrity, avoid repeated cycles of freezing and thawing.

Purity

The purity of the PNRC2 protein is determined using SDS-PAGE analysis and is guaranteed to be greater than 90%.

Synonyms

Proline-rich nuclear receptor coactivator 2, PNRC2.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMGGGERY NIPAPQSRNV SKNQQQLNRQ KTKEQNSQMK IVHKKKERGH GYNSSAAAWQ AMQNGGKNKN FPNNQSWNSS LSGPRLLFKS QANQNYAGAK FSEPPSPSVL PKPPSHWVPV SFNPSDKEIM TFQLKTLLKV QV.

Product Science Overview

Structure and Function

PNRC2 is a relatively small coactivator with a molecular weight of approximately 16 kDa. It is characterized by a proline-rich sequence, which is essential for its interaction with nuclear receptors. The protein contains an SH3 domain-binding motif (SEPPSPS) and an NR box-like sequence (LKTLL), which are critical for its function.

PNRC2 interacts with nuclear receptors in both ligand-dependent and ligand-independent manners. It has been shown to interact with orphan receptors such as steroidogenic factor 1 (SF1) and estrogen receptor-related receptor α1, as well as with the ligand-binding domains of various other nuclear receptors, including estrogen receptor, glucocorticoid receptor, progesterone receptor, thyroid receptor, retinoic acid receptor, and retinoid X receptor.

Role in mRNA Decay

One of the significant functions of PNRC2 is its involvement in nonsense-mediated mRNA decay (NMD). NMD is a surveillance pathway that degrades mRNAs containing premature stop codons, preventing the production of truncated and potentially harmful proteins. PNRC2 acts as a bridge between the mRNA decapping complex and the NMD machinery, facilitating the degradation of aberrant mRNAs . It is required for the localization of UPF1/RENT1 to the P-body, a cellular structure involved in mRNA decay.

Interaction with Glucocorticoid Receptor

PNRC2 also plays a role in glucocorticoid receptor-mediated mRNA degradation. It interacts with the glucocorticoid receptor (NR3C1) in a ligand-dependent manner when the receptor is bound to the 5’ untranslated region (UTR) of target mRNAs. This interaction recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay.

Clinical Significance

Mutations or dysregulation of the PNRC2 gene have been associated with various diseases. For instance, PNRC2 is linked to Leydig Cell Hypoplasia, Type I, and Autosomal Recessive Limb-Girdle Muscular Dystrophy Type 2W. Additionally, PNRC2 is involved in pathways related to viral mRNA translation and the processing of capped intron-containing pre-mRNA.

Research and Applications

Research on PNRC2 continues to uncover its diverse roles in cellular processes and its potential implications in disease. The recombinant form of PNRC2 is used in various experimental setups to study its function and interactions with other proteins. Understanding the mechanisms by which PNRC2 operates can provide insights into the regulation of gene expression and the maintenance of cellular homeostasis.

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PNRC2 Human

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