PNPT1 Human
Polyribonucleotide Nucleotidyltransferase 1 Human Recombinant
Cat. No.
BT12082
Source
Escherichia Coli.
Synonyms
Polyribonucleotide Nucleotidyltransferase 1, Polynucleotide
Phosphorylase-Like Protein, Polynucleotide Phosphorylase 1, 3-5 RNA Exonuclease
OLD35, PNPase Old-35, EC 2.7.7.8, PNPase 1, COXPD13, DFNB70, PNPASE, OLD35, Polyribonucleotide
Nucleotidyltransferase 1, Mitochondrial, Deafness, Autosomal Recessive 70, Polynucleotide
Phosphorylase, 3-5 RNA Exonuclease, EC 2.7.7, Old-35, Polyribonucleotide
nucleotidyltransferase 1, mitochondrial, 3'-5' RNA exonuclease OLD35, PNPase
old-35.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
PNPT1 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 761 amino acids (46-783 a.a) and having a
molecular mass of 83.3kDa.
PNPT1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Product Specs
Introduction
Polyribonucleotide nucleotidyltransferase 1, also known as PNPT1, is a protein primarily located in the mitochondrial intermembrane space. It plays a crucial role in transporting RNA into mitochondria. Genetic mutations in PNPT1 have been linked to combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Pseudogenes related to PNPT1 have been identified on chromosomes 3 and 7.
Description
Recombinant human PNPT1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 761 amino acids (46-783 a.a.). It has a molecular mass of 83.3 kDa. The protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
PNPT1 protein solution at a concentration of 0.25 mg/ml in phosphate buffered saline (pH 7.4) with 10% glycerol and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 85.0% as determined by SDS-PAGE.
Synonyms
Polyribonucleotide Nucleotidyltransferase 1, Polynucleotide
Phosphorylase-Like Protein, Polynucleotide Phosphorylase 1, 3-5 RNA Exonuclease
OLD35, PNPase Old-35, EC 2.7.7.8, PNPase 1, COXPD13, DFNB70, PNPASE, OLD35, Polyribonucleotide
Nucleotidyltransferase 1, Mitochondrial, Deafness, Autosomal Recessive 70, Polynucleotide
Phosphorylase, 3-5 RNA Exonuclease, EC 2.7.7, Old-35, Polyribonucleotide
nucleotidyltransferase 1, mitochondrial, 3'-5' RNA exonuclease OLD35, PNPase
old-35.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSAVAVDLG NRKLEISSGK LARFADGSAV VQSGDTAVMV TAVSKTKPSP
SQFMPLVVDY RQKAAAAGRI PTNYLRREIG TSDKEILTSR IIDRSIRPLF PAGYFYDTQV LCNLLAVDGV
NEPDVLAING ASVALSLSDI PWNGPVGAVR IGIIDGEYVV NPTRKEMSSS TLNLVVAGAP KSQIVMLEAS
AENILQQDFC HAIKVGVKYT QQIIQGIQQL VKETGVTKRT PQKLFTPSPE IVKYTHKLAM ERLYAVFTDY
EHDKVSRDEA VNKIRLDTEE QLKEKFPEAD PYEIIESFNV VAKEVFRSIV LNEYKRCDGR DLTSLRNVSC
EVDMFKTLHG SALFQRGQTQ VLCTVTFDSL ESGIKSDQVI TAINGIKDKN FMLHYEFPPY ATNEIGKVTG
LNRRELGHGA LAEKALYPVI PRDFPFTIRV TSEVLESNGS SSMASACGGS LALMDSGVPI SSAVAGVAIG
LVTKTDPEKG EIEDYRLLTD ILGIEDYNGD MDFKIAGTNK GITALQADIK LPGIPIKIVM EAIQQASVAK
KEILQIMNKT ISKPRASRKE NGPVVETVQV PLSKRAKFVG PGGYNLKKLQ AETGVTISQV DEETFSVFAP
TPSAMHEARD FITEICKDDQ EQQLEFGAVY TATITEIRDT GVMVKLYPNM TAVLLHNTQL DQRKIKHPTA
LGLEVGQEIQ VKYFGRDPAD GRMRLSRKVL QSPATTVVRT LNDRSSIVMG EPISQSSSNS Q.
Product Science Overview
Structure and Localization
PNPT1 is a phosphate-dependent 3’-to-5’ exoribonuclease that catalyzes the phosphorolysis of single-stranded polyribonucleotides in a processive manner . The enzyme is predominantly localized in the mitochondrial intermembrane space, where it is involved in the import of RNA into mitochondria . The human recombinant form of PNPT1 is produced in E. coli and consists of a single, non-glycosylated polypeptide chain containing 761 amino acids, with a molecular mass of approximately 83.3 kDa .
Function
PNPT1 is a component of the mitochondrial degradosome (mtEXO) complex, which degrades 3’ overhang double-stranded RNA in an ATP-dependent manner . This enzyme is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules . Additionally, PNPT1 has been implicated in numerous RNA metabolic processes, including RNA processing and degradation .
Clinical Significance
Research and Applications
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