PNPLA2 Antibody
Patatin-like phospholipase domain-containing protein 2, Adipose triglyceride lipase, Desnutrin Transport-secretion protein 2, TTS2.2, Calcium-independent phospholipase A2, IPLA2-zeta Pigment epithelium-derived factor, TTS2, PNPLA2, ATGL, PEDF-R, FP17548, TTS-2.2, DKFZp667M109, 1110001C14Rik.
Description
Product Specs
PNPLA2, also known as Adipose Triglyceride Lipase (ATGL), initiates the breakdown of triglycerides into fatty acids within adipose tissue. Genetic alterations in the PNPLA2 gene have been associated with a medical condition known as neutral lipid storage disease with myopathy.
A clear and colorless solution that has undergone sterile filtration.
The antibody is supplied as a 1 mg/ml solution in a buffer consisting of phosphate-buffered saline (PBS) at a pH of 7.4, 10% glycerol, and 0.02% sodium azide.
For short-term storage (up to 1 month), maintain the antibody at 4°C. For extended storage, it is recommended to store the antibody at -20°C. Avoid repeated freeze-thaw cycles to preserve antibody stability.
The antibody remains stable for a period of 12 months when stored at -20°C. At a temperature of 4°C, the antibody can be stored for up to 1 month.
This PNPLA2 antibody has undergone rigorous validation in various applications, including ELISA, Western blotting, flow cytometry, and immunocytochemistry/immunofluorescence (ICC/IF), demonstrating its specificity and reactivity. As optimal working concentrations may vary depending on the specific application and experimental conditions, it is highly recommended to perform a titration to determine the most suitable concentration for your particular experiment.
Patatin-like phospholipase domain-containing protein 2, Adipose triglyceride lipase, Desnutrin Transport-secretion protein 2, TTS2.2, Calcium-independent phospholipase A2, IPLA2-zeta Pigment epithelium-derived factor, TTS2, PNPLA2, ATGL, PEDF-R, FP17548, TTS-2.2, DKFZp667M109, 1110001C14Rik.
PNPLA2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT18E6AT.
Anti-human PNPLA2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PNPLA2 protein 30-504 amino acids purified from E. coli.
Mouse IgG2b heavy chain and k light chain.
Product Science Overview
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins . These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins . They are commonly used in various applications, including detection, sorting, and purification of human proteins .
Applications
Mouse anti-human antibodies are versatile tools in biomedical research. They can be conjugated with various labels such as horseradish peroxidase (HRP), alkaline phosphatase (AP), and fluorescent dyes to facilitate detection in different assays . These antibodies are widely used in techniques like ELISA, Western blotting, immunohistochemistry, and flow cytometry .
HAMA Response
One potential issue with the use of mouse anti-human antibodies is the development of human anti-mouse antibodies (HAMA) in patients . The HAMA response can range from mild allergic reactions to severe, life-threatening conditions .
