PMF1 is involved in the regulation of polyamine levels within cells. Polyamines are organic compounds that play critical roles in cellular functions such as DNA stabilization, gene expression, and cell proliferation. PMF1 is known to interact with other proteins to modulate the activity of polyamines, thereby influencing various cellular processes.
BGLAP, commonly known as Osteocalcin, is a protein that is highly expressed in bone tissue. It plays a crucial role in bone mineralization and calcium ion homeostasis. Osteocalcin is produced by osteoblasts and is involved in the regulation of bone formation and remodeling. It also has roles in energy metabolism and male fertility.
The PMF1-BGLAP fusion protein is a result of read-through transcription, where the transcription machinery continues from the PMF1 gene into the BGLAP gene without terminating. This fusion protein retains functional domains from both parent proteins, potentially combining their roles in polyamine regulation and bone metabolism .
The recombinant PMF1-BGLAP protein is typically produced in Escherichia coli (E. coli). The protein is expressed as a single, non-glycosylated polypeptide chain containing 234 amino acids, with a molecular mass of approximately 26.2 kDa . It is often fused to a His-tag at the N-terminus to facilitate purification using affinity chromatography techniques .
Recombinant PMF1-BGLAP protein is used in various research applications, including:
The recombinant PMF1-BGLAP protein is typically stored in a buffer containing Tris-HCl and glycerol to maintain stability. It can be stored at +4°C for short-term use (1-2 weeks) or at -20°C to -80°C for long-term storage. It is important to avoid repeated freeze-thaw cycles to preserve protein integrity .