PLEKHF2 Human

Pleckstrin Homology Domain Containing Family F Member 2 Human Recombinant
Cat. No.
BT7817
Source
Escherichia Coli.
Synonyms
EAPF, PHAFIN2, ZFYVE18, Pleckstrin homology domain-containing family F member 2, PH domain-containing family F member 2, Endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains, PH and FYVE domain-containing protein 2, Zinc finger FYVE domain-containing protein 18, PLEKHF2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PLEKHF2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 272 amino acids (1-249 a.a) and having a molecular mass of 30.2kDa.
PLEKHF2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Pleckstrin Homology Domain Containing Family F Member 2, also known as PLEKHF2, is a protein found in humans. It plays a role in the early stages of endosome fusion, a process important for transporting molecules within cells. PLEKHF2 contains specific regions called FYVE and PH domains, which are involved in binding to other molecules. This protein is important for regulating receptor trafficking, fluid-phase transport, and the cell's sensitivity to TNF-induced apoptosis, a type of programmed cell death.
Description
This product consists of the human PLEKHF2 protein, manufactured in E. coli bacteria. It is a single, non-glycosylated polypeptide chain comprising 272 amino acids (specifically, amino acids 1 through 249). This protein has a molecular weight of 30.2 kDa. To facilitate purification and identification, a 23 amino acid His-tag is attached to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear and colorless solution that has been sterilized through filtration.
Formulation
This solution contains 0.25mg/ml of PLEKHF2 protein. It is buffered with 20mM Tris-HCl at a pH of 8.0. Additionally, it includes 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
The product can be stored at 4°C for 2-4 weeks. For longer storage, freezing at -20°C is recommended. Adding a carrier protein such as HSA or BSA (0.1%) is advisable for extended storage. Repeated freezing and thawing should be avoided.
Purity
The purity of this product is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
EAPF, PHAFIN2, ZFYVE18, Pleckstrin homology domain-containing family F member 2, PH domain-containing family F member 2, Endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains, PH and FYVE domain-containing protein 2, Zinc finger FYVE domain-containing protein 18, PLEKHF2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVDRLAN SEANTRRISI VENCFGAAGQ PLTIPGRVLI GEGVLTKLCR KKPKARQFFL FNDILVYGNI VIQKKKYNKQ HIIPLENVTI DSIKDEGDLR NGWLIKTPTK SFAVYAATAT EKSEWMNHIN KCVTDLLSKS GKTPSNEHAA VWVPDSEATV CMRCQKAKFT PVNRRHHCRK CGFVVCGPCS EKRFLLPSQS SKPVRICDFC YDLLSAGDMA TCQPARSDSY SQSLKSPLND MSDDDDDDDS SD.

Product Science Overview

Structure and Domains

The PH domain is a protein domain of approximately 120 amino acids that occurs in a wide range of proteins involved in intracellular signaling or as constituents of the cytoskeleton . This domain can bind phosphatidylinositol lipids within biological membranes, playing a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways .

The FYVE domain is a zinc finger domain that specifically binds phosphatidylinositol 3-phosphate (PI3P), a phosphoinositide found in cellular membranes. This binding is essential for the localization of FYVE domain-containing proteins to endosomal membranes, where they participate in membrane trafficking and signal transduction.

Function

PLEKHF2 is predicted to enable phosphatidylinositol binding activity and is involved in protein transport . It plays a role in early endosome fusion upstream of RAB5, thereby regulating receptor trafficking and fluid-phase transport . Additionally, PLEKHF2 enhances cellular sensitivity to tumor necrosis factor (TNF)-induced apoptosis .

Biological Significance

The ability of PLEKHF2 to bind phosphatidylinositol lipids and its involvement in endosomal trafficking highlight its importance in maintaining cellular homeostasis and signaling. Dysregulation of these processes can lead to various diseases, including cancer and neurodegenerative disorders.

Research and Applications

Human recombinant PLEKHF2 is used in research to study its role in cellular processes and its potential as a therapeutic target. Understanding the function and regulation of PLEKHF2 can provide insights into the mechanisms of diseases and aid in the development of novel therapeutic strategies.

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