Pleiotrophin Human, His
Pleiotrophin Human Recombinant, His Tag
Cat. No.
BT11921
Source
Escherichia Coli.
Synonyms
PTN, Heparin Affin Regulatory Protein, HARP, Heparin-binding growth factor-8, HBGF-8, Osteoblast-Specific Factor-1, OSF-1, Heparin-binding growth-associated molecule, HB-GAM, HBNF-1 Heparin-binding brain mitogen, Heparin-binding neurite outgrowth-promoting factor 1, HBBM, NEGF1.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Pleiotrophin Human Recombinant contains His-Tagged Fusion Protein, produced in E. coli, its molecular weight is 17.3 kDa protein containing 136 amino acid residues of the OSF-1 human and 16 additional amino acid residues - HisTag, thrombin cleavage site (underlined).
Product Specs
Introduction
Pleiotrophin, also known as Osteoblast-Specific Factor-1 (OSF-1), is a protein comprised of 136 amino acid residues. Its structure is notable for a high proportion of cationic amino acids (24%), with lysine clusters located at both the N-terminal and C-terminal ends. Gene expression analysis using Northern blotting revealed that the OSF-1 gene is expressed in mouse calvarial osteoblast-enriched cells and brain tissues. However, it was not detected in other tissues such as the thymus, spleen, kidney, liver, lung, testis, or heart. Pleiotrophin demonstrates the ability to stimulate adhesion, migration, proliferation, and differentiation in human osteoprogenitor cells. This embryonic growth and differentiation factor is not only present in certain types of cancer but also found in adults experiencing inflammatory diseases. Notably, in osteoarthritis, pleiotrophin is particularly prominent in the early stages, and its concentration in the synovial fluid could potentially serve as a marker for disease progression. Pleiotrophin might play a role in cartilage repair within the context of osteoarthritis, especially during its initial phases.
Description
Recombinant Human Pleiotrophin, a His-Tagged Fusion Protein, is produced in E. coli. This 17.3 kDa protein comprises 136 amino acid residues of human OSF-1 and an additional 16 amino acid residues, including a HisTag and a thrombin cleavage site.
Physical Appearance
White lyophilized powder after filtration.
Formulation
The protein solution, at a concentration of 0.5 mg/ml in 0.1M phosphate buffer with 0.1M NaCl at pH 7.2, is filtered and then lyophilized.
Solubility
To reconstitute, add PBS at pH 7.2 and allow the lyophilized pellet to dissolve completely.
Stability
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to minimize repeated freezing and thawing. The reconstituted protein remains stable at 4°C for a limited period and shows no significant change after two weeks at this temperature.
Purity
Purity is determined to be greater than 95% using SDS-PAGE analysis.
Synonyms
PTN, Heparin Affin Regulatory Protein, HARP, Heparin-binding growth factor-8, HBGF-8, Osteoblast-Specific Factor-1, OSF-1, Heparin-binding growth-associated molecule, HB-GAM, HBNF-1 Heparin-binding brain mitogen, Heparin-binding neurite outgrowth-promoting factor 1, HBBM, NEGF1.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHHM LVPRGSGKKE KPEKKVKKSD CGEWQWSVCV PTSGDCGLGT REGTRTGAEC KQTMKTQRCK IPCNWKKQFG AECKYQFQAW GECDLNTALK TRTGSLKRAL HNAECQKTVT ISKPCGKLTK PKPQAESKKK KKEGKKQEKM LD.
Product Science Overview
Biological Significance
Pleiotrophin plays a crucial role in various biological processes:
- Neural Development: It stimulates neural development and provides trophic support, promoting positive recovery in neurodegenerative disorders .
- Hematopoietic Stem Cell Maintenance: PTN is involved in the maintenance of hematopoietic stem cells .
- Cell Differentiation: It aids in cell differentiation, contributing to the development and function of various tissues .
High serum levels of pleiotrophin are associated with a variety of solid tumors, including multiple myeloma . However, its expression is very limited in normal adult tissues .
Recombinant Pleiotrophin
Recombinant pleiotrophin is produced using Escherichia coli (E. coli) expression systems . The recombinant protein is often tagged with a His tag to facilitate purification and detection. The His tag is a sequence of histidine residues that binds to nickel ions, allowing for easy isolation of the protein using nickel-affinity chromatography.
Applications
Recombinant pleiotrophin is used in various research applications, including:
Preparation and Storage
The lyophilized recombinant pleiotrophin is reconstituted in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. It is recommended to store the reconstituted protein at 2°C to 8°C for up to one week. For extended storage, it should be further diluted in a buffer containing a carrier protein, such as 0.1% bovine serum albumin (BSA), and stored in working aliquots at -20°C to -80°C .
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