PLDN Human

Pallidin homolog, also known as PLDN, is a protein that plays a crucial role in intracellular vesicle trafficking. This protein is encoded by the BLOC1S6 gene and is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules .

The recombinant human pallidin protein is typically produced in Escherichia coli (E. coli) and includes an N-terminal His-tag. The amino acid sequence of this recombinant protein corresponds to the amino acids 1-172 of the human pallidin protein . The theoretical molecular weight of the protein is approximately 21.9 kDa, although the observed molecular weight may vary due to post-translational modifications and other experimental factors .

Pallidin interacts with Syntaxin 13, a protein that mediates intracellular membrane fusion. This interaction is essential for the proper functioning of intracellular vesicle trafficking. The protein is also involved in the development of lysosome-related organelles, which are crucial for various cellular processes .

Recombinant human pallidin is used in various research applications, including:

• Western Blotting: To detect the presence of pallidin in different cell lines.
• Immunohistochemistry: To study the localization of pallidin in tissue samples.
• Flow Cytometry: To analyze the expression of pallidin in different cell populations .

The recombinant human pallidin protein should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain the protein’s stability. The protein is typically supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 2 mM DTT, 10% glycerol, and 100 mM NaCl .