PLAUR Human

PLAUR Human Recombinant
Cat. No.
BT7606
Source
Sf9, Baculovirus cells.
Synonyms

PLAUR, Monocyte Activation Antigen Mo3, U-PAR, UPAR, U-Plasminogen Activator Receptor Form 2, CD87 Antigen , CD87, URKR, MO3.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PLAUR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 291 amino acids (23-305 a.a.) and having a molecular mass of 32.5kDa (Molecular size on SDS-PAGE will appear at approximately 40-57 kDa).
PLAUR is fused to a 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The urokinase-type plasminogen activator receptor, also known as uPAR, is encoded by the PLAUR gene in humans. uPAR is one of two activators (the other being tPA) that convert the extracellular zymogen plasminogen to plasmin, a serine protease involved in various normal and pathological processes that require cell migration and/or tissue destruction. PLAUR protein is synthesized and released from cells as a single-chain proenzyme with narrow enzymatic activity. It is converted to an active two-chain disulfide-linked active enzyme by plasmin and other specific proteinases.
Description
PLAUR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 291 amino acids (23-305 a.a.) and having a molecular mass of 32.5 kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57 kDa). PLAUR is fused to an 8 amino acid His-tag at the C-terminus and purified by proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The PLAUR protein solution (0.5 mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if the entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms

PLAUR, Monocyte Activation Antigen Mo3, U-PAR, UPAR, U-Plasminogen Activator Receptor Form 2, CD87 Antigen , CD87, URKR, MO3.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence
LRCMQCKTNG DCRVEECALG QDLCRTTIVR LWEEGEELEL VEKSCTHSEK TNRTLSYRTG LKITSLTEVV CGLDLCNQGN SGRAVTYSRS RYLECISCGS SDMSCERGRH QSLQCRSPEE QCLDVVTHWI QEGEEGRPKD DRHLRGCGYL PGCPGSNGFH NNDTFHFLKC CNTTKCNEGP ILELENLPQN GRQCYSCKGN STHGCSSEET FLIDCRGPMN QCLVATGTHE PKNQSYMVRG CATASMCQHA HLGDAFSMNH IDVSCCTKSG CNHPDLDVQY RSGLEHHHHH H.

Product Science Overview

Structure and Function

PLAUR is a highly glycosylated protein with a molecular weight of approximately 55-60 kDa. It is anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor . The primary function of PLAUR is to bind to urokinase plasminogen activator (uPA), facilitating the conversion of plasminogen to plasmin, a serine protease involved in the degradation of the extracellular matrix and fibrinolysis .

Role in Cancer

PLAUR is significantly implicated in cancer biology. Overexpression of PLAUR and uPA is strongly correlated with poor prognosis in various malignant tumors . The uPA/PLAUR system drives tumor progression by mediating directed extracellular proteolysis on the surface of migrating or invading cells . Targeting PLAUR has been proposed as a novel approach for inhibiting tumor progression, making it a potential therapeutic target in cancer treatment .

Recombinant PLAUR

Recombinant PLAUR (Human) is produced using DNA sequences encoding the human UPAR isoform 1 (Q03405-1) expressed in HEK293 cells . The recombinant protein is typically tagged with a polyhistidine tag for purification purposes and is used in various research applications, including functional assays and structural studies .

Expression and Localization

PLAUR is expressed in various tissues, with high cytoplasmic expression in respiratory epithelia and a subset of cells in the bone marrow . It is predominantly localized to the plasma membrane but can also be found intracellularly in different isoforms .

Clinical Significance

The levels of PLAUR and uPA in both resected tumor tissue and plasma are of independent prognostic significance for patient survival in several types of human cancer . This makes PLAUR a valuable biomarker for cancer prognosis and a potential target for therapeutic intervention.

Research and Applications

Recombinant PLAUR is widely used in research to study its role in cancer and other diseases. Functional assays, such as ELISA, are employed to measure its binding ability and interactions with other proteins . Additionally, PLAUR’s involvement in the tumor microenvironment and its interaction with tumor-associated macrophages (TAMs) and glioma cells have been subjects of recent studies .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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