PKNOX1 Human

PBX/Knotted 1 Homeobox 1 Human Recombinant
Cat. No.
BT7436
Source
Escherichia Coli.
Synonyms
pkonx1c, PREP1, Homeobox protein PKNOX1, Homeobox protein PREP-1, PBX/knotted homeobox 1, PKNOX1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PKNOX1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 456 amino acids (1-436 a.a) and having a molecular mass of 49.7kDa.
PKNOX1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PBX/Knotted 1 Homeobox 1 (PKNOX1), also known as UEF3, PRP-1, and p64, is a DNA-binding Hmeobox protein involved in regulating the transcriptional activity of promoters containing AP-1. It forms stable complexes with PBX proteins, synergizing with AP-1 binding factors.
Description
Recombinant human PKNOX1, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 456 amino acids (1-436 a.a) with a molecular weight of 49.7 kDa. The protein includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
PKNOX1 protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution contains 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store the protein solution at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of PKNOX1 protein is greater than 80% as determined by SDS-PAGE analysis.
Synonyms
pkonx1c, PREP1, Homeobox protein PKNOX1, Homeobox protein PREP-1, PBX/knotted homeobox 1, PKNOX1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MMATQTLSID SYQDGQQMQV VTELKTEQDP NCSEPDAEGV SPPPVESQTP MDVDKQAIYR HPLFPLLALL FEKCEQSTQG SEGTTSASFD VDIENFVRKQ EKEGKPFFCE DPETDNLMVK AIQVLRIHLL ELEKVNELCK DFCSRYIACL KTKMNSETLL SGEPGSPYSP VQSQQIQSAI TGTISPQGIV VPASALQQGN VAMATVAGGT VYQPVTVVTP QGQVVTQTLS PGTIRIQNSQ LQLQLNQDLS ILHQDDGSSK NKRGVLPKHA TNVMRSWLFQ HIGHPYPTED EKKQIAAQTN LTLLQVNNWF INARRRILQP MLDSSCSETP KTKKKTAQNR PVQRFWPDSI ASGVAQPPPS ELTMSEGAVV TITTPVNMNV DSLQSLSSDG ATLAVQQVMM AGQSEDESVD STEEDAGALA PAHISGLVLE NSDSLQ.

Product Science Overview

Gene and Protein Structure

The PKNOX1 gene is located on chromosome 21q22.3 in humans . It encodes a homeodomain-containing polypeptide of 436 amino acids . The protein has a calculated molecular mass of approximately 49 kDa . The homeodomain is a conserved DNA-binding domain that allows the protein to bind to specific DNA sequences and regulate the transcription of target genes .

Expression and Function

PKNOX1 is expressed in various human tissues, including the adult heart, brain, placenta, lung, liver, muscle, kidney, and pancreas, as well as in several fetal tissues . The protein plays a significant role in the formation of transcriptionally active complexes that are involved in development and organogenesis .

PKNOX1 forms complexes with other proteins, such as PBX1 and PBX2, to regulate gene expression. These complexes recognize specific DNA sequences and modulate the transcription of target genes . The interaction of PKNOX1 with its partners is independent of DNA, indicating that the protein-protein interactions are crucial for its function .

Biological Significance

PKNOX1 is involved in several biological processes, including angiogenesis, regulation of transcription by RNA polymerase II, camera-type eye development, hemopoiesis, and positive regulation of transcription . The protein is predicted to be located in both the cytoplasm and nucleus and is part of chromatin .

Clinical Relevance

Mutations or dysregulation of PKNOX1 have been associated with various diseases. For example, PKNOX1 is linked to familial temporal lobe epilepsy . Understanding the function and regulation of PKNOX1 can provide insights into the molecular mechanisms underlying these diseases and potentially lead to the development of targeted therapies.

Research and Applications

Recombinant PKNOX1 protein is used in research to study its function and interactions with other proteins. By expressing and purifying the recombinant protein, researchers can investigate its role in gene regulation and its potential involvement in disease processes. This knowledge can contribute to the development of new therapeutic strategies for diseases associated with PKNOX1 dysregulation.

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