PKA-RII alpha
Description
Protein Kinase A is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
cAMP-Dependent Protein Kinase A (PKA) is a crucial enzyme in cellular signaling pathways. It is also known as Protein Kinase A and is involved in regulating various cellular processes, including metabolism, gene expression, and cell cycle progression. The enzyme’s activity is dependent on the levels of cyclic adenosine monophosphate (cAMP) within the cell. PKA is composed of two main subunits: the regulatory subunits and the catalytic subunits. The focus of this article is on the regulatory subunit-II A (RIIA) and its recombinant form.
PKA exists as a tetrameric holoenzyme consisting of two regulatory subunits and two catalytic subunits. The regulatory subunits are responsible for maintaining the enzyme in an inactive state by binding to the catalytic subunits. Upon binding of cAMP to the regulatory subunits, a conformational change occurs, leading to the release and activation of the catalytic subunits .
There are two major types of regulatory subunits: type I (RI) and type II (RII). The RII subunits are further divided into RIIα and RIIβ. The RIIα subunit, encoded by the PRKAR2A gene, is the focus of this article. The RIIα subunit plays a critical role in determining the subcellular localization of PKA and its interaction with various A-kinase anchoring proteins (AKAPs) .
The recombinant form of the RIIα subunit is produced using recombinant DNA technology, which involves inserting the gene encoding the RIIα subunit into an expression vector and introducing it into a host cell for protein production. This recombinant protein is used in various research applications to study the structure, function, and regulation of PKA.
The RIIα subunit is involved in several important cellular processes. It regulates protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). Additionally, it plays a role in the regulation of lipid and glucose metabolism, as well as the signal transduction mechanisms of certain G protein-coupled receptors (GPCRs) .
Mutations in the PRKAR2A gene, which encodes the RIIα subunit, have been associated with various diseases, including Carney Complex Variant and Macs Syndrome . Understanding the function and regulation of the RIIα subunit is crucial for developing potential therapeutic strategies for these conditions.
