PIP4K2B Human

Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, Beta Human Recombinant
Cat. No.
BT13494
Source
Escherichia Coli.
Synonyms
PI5P4KB, PIP5K2B, PIP5KIIB, PIP5KIIbeta, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PtdIns(5)P-4-kinase isoform 2-beta, PIP4K2B.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PIP4K2B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 439 amino acids (1-416 a.a) and having a molecular mass of 49.8kDa.PIP4K2B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, Beta (PIP4K2B) is a member of the phosphatidylinositol-4-phosphate 5-kinase family. This enzyme catalyzes the phosphorylation of phosphatidylinositol-4-phosphate, specifically at the 5th hydroxyl group of the myo-inositol ring. This reaction results in the formation of phosphatidylinositol-4,5-bisphosphate. Although PIP4K2B exhibits kinase activity, its amino acid sequence lacks similarity to other known kinases. Additionally, PIP4K2B has been found to interact with the p55 TNF receptor.
Description
Recombinant PIP4K2B from humans has been produced in E. coli. This protein is a single, non-glycosylated polypeptide chain consisting of 439 amino acids (specifically, amino acids 1-416). It has a molecular mass of 49.8 kDa. The protein includes a 23 amino acid His-tag fused to its N-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The PIP4K2B protein solution is provided at a concentration of 0.5 mg/ml. The solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to store the product frozen at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). It is important to avoid subjecting the product to multiple freeze-thaw cycles.
Purity
The purity of the protein is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms
PI5P4KB, PIP5K2B, PIP5KIIB, PIP5KIIbeta, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PtdIns(5)P-4-kinase isoform 2-beta, PIP4K2B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSNCTS TTAVAVAPLS ASKTKTKKKH FVCQKVKLFR ASEPILSVLM WGVNHTINEL SNVPVPVMLM PDDFKAYSKI KVDNHLFNKE NLPSRFKFKE YCPMVFRNLR ERFGIDDQDY QNSVTRSAPI NSDSQGRCGT RFLTTYDRRF VIKTVSSEDV AEMHNILKKY HQFIVECHGN TLLPQFLGMY RLTVDGVETY MVVTRNVFSH RLTVHRKYDL KGSTVAREAS DKEKAKDLPT FKDNDFLNEG QKLHVGEESK KNFLEKLKRD VEFLAQLKIM DYSLLVGIHD VDRAEQEEME VEERAEDEEC ENDGVGGNLL CSYGTPPDSP GNLLSFPRFF GPGEFDPSVD VYAMKSHESS PKKEVYFMAI IDILTPYDTK KKAAHAAKTV KHGAGAEIST VNPEQYSKRF NEFMSNILT.

Product Science Overview

Introduction

Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, Beta (PIP4K2B) is a member of the phosphatidylinositol-5-phosphate 4-kinase family. These enzymes play a crucial role in the phosphorylation of phosphatidylinositol 5-phosphate (PI5P) at the fourth position of the inositol ring, leading to the synthesis of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) .

Structure and Function

PIP4K2B is a protein-coding gene that is involved in various cellular processes. The enzyme has a low enzymatic activity and may act as a GTP sensor, exhibiting higher GTP-dependent kinase activity than ATP-dependent kinase activity . PIP4K2B negatively regulates insulin signaling through a catalytic-independent mechanism .

Cellular Role

PIP4K2B is integral to several cellular processes, including growth, development, metabolism, and tumor growth . It is overexpressed in certain cancers, highlighting its potential as a therapeutic target . The enzyme’s role in regulating proteins involved in genome stability and cell homeostasis underscores its importance in maintaining cellular integrity .

Research and Applications

Recent studies have shown that PIP4K2B, along with its isoforms PIP4K2A and PIP4K2C, is central to phosphoinositide signaling, which regulates cellular processes under stress and physiological conditions . Proteome profiling of PIP4K2B knockdown cells has identified modifications in key regulators involved in cell homeostasis and genome integrity . These findings suggest that PIP4K2B could be a promising therapeutic target for cells experiencing genotoxic stress conditions .

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