PINX1 Human
Description
PINX1 is fused to 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
MGSSHHHHHH SSGLVPRGSH MSMLAERRRK QKWAVDPQNT AWSNDDSKFG QRMLEKMGWS KGKGLGAQEH GATDHIKVQV KNNHLGLGAT INNEDNWIAH QDDFNQLLAE LNTCHGQETT DSSDKKEKKS FSLEEKSKIS KNRVHYMKFT KGKDLSSRSK TDLDCIFGKR QSKKTPEGDA SPSTPEENET TTTSAFTIQE YFAKRMAALK NKPQVPVPGS DISETQVERK RGKKINKEAT GKDVESYLQP KAKRHTEGKP ERAEAQERVA KKKSAPAEEQ LRGPCWDQSS KASAQDAGDH VQPPEGRDFT LKPKKRRGKK KLQKPVEIAE DATLEETLVK KKKKKDSK.
Product Science Overview
PINX1 contains a telomerase inhibitory domain (TID), which is essential for its function. There are two known variants of PINX1, with the second variant lacking an exon in the 3’ coding region, resulting in a frameshift compared to variant 1. This frameshift leads to a shorter isoform with a distinct C-terminus .
PINX1 interacts with several key proteins, including TERF1 (Telomeric Repeat-binding Factor 1), MCRS1 (Microspherule Protein 1), and TERT (Telomerase Reverse Transcriptase). By binding to these proteins, PINX1 can effectively inhibit telomerase activity, leading to telomere shortening and induction of cellular crisis. Over-expression of PINX1 results in decreased telomerase activity, while reduction of PINX1 leads to increased telomerase activity and elongation of telomeres .
Recombinant human PINX1 is produced using E. coli expression systems and is often tagged with a His-tag for purification purposes. The recombinant protein is typically used in research to study its function and interactions with other proteins. It is stored in a buffer containing Tris-HCl, DTT, and glycerol to maintain stability .
