PIM2 Human
PIM2 Human Recombinant
Cat. No.
BT4492
Source
Escherichia Coli.
Synonyms
Pim-2 oncogene, Serine/threonine-protein kinase pim-2, Pim-2h, PIM2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
PIM2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 334 amino acids (1-311 a.a) and having a molecular mass of 36.6kDa.
PIM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PIM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
PIM2, a member of the CAMK family, is a serine/threonine protein kinase. Its expression is observed in various tissues, particularly in the spleen, thymus, testis, and small intestine. PIM2 plays a role in tumor development and is involved in the establishment and maintenance of Long-Term Potentiation (LTP). Furthermore, it contributes to the positive regulation of chondrocyte survival and autophagy within the epiphyseal growth plate.
Description
Recombinant PIM2 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 334 amino acids (amino acids 1-311). It has a molecular weight of 36.6 kDa. The protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A sterile, filtered solution that is colorless.
Formulation
PIM2 protein solution at a concentration of 0.25 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
Purity is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
Pim-2 oncogene, Serine/threonine-protein kinase pim-2, Pim-2h, PIM2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLTKPLQ GPPAPPGTPT PPPGGKDREA FEAEYRLGPL LGKGGFGTVF AGHRLTDRLQ VAIKVIPRNR VLGWSPLSDS VTCPLEVALL WKVGAGGGHP GVIRLLDWFE TQEGFMLVLE RPLPAQDLFD YITEKGPLGE GPSRCFFGQV VAAIQHCHSR GVVHRDIKDE NILIDLRRGC AKLIDFGSGA LLHDEPYTDF DGTRVYSPPE WISRHQYHAL PATVWSLGIL LYDMVCGDIP FERDQEILEA ELHFPAHVSP DCCALIRRCL APKPSSRPSL EEILLDPWMQ TPAEDVPLNP SKGGPAPLAW SLLP.
Product Science Overview
Structure and Expression
PIM2 is a proto-oncogene with serine/threonine kinase activity involved in cell survival and proliferation . It is expressed in various tissues and is particularly elevated in multiple myeloma (MM) cells . The human recombinant PIM2 protein is typically expressed in baculovirus-infected Sf9 cells and has a molecular weight of approximately 35.5 kDa .
Function and Mechanism
PIM2 plays a crucial role in several cellular processes:
- Cell Survival and Proliferation: PIM2 promotes cell survival and proliferation by regulating MYC transcriptional activity, cell cycle progression, and cap-dependent protein translation .
- Phosphorylation of BAD: PIM2 phosphorylates the pro-apoptotic protein BAD, thereby promoting cell survival .
- mTOR-C1 Pathway: PIM2 phosphorylates TSC2, a negative regulator of mTOR-C1, on Ser-1798. This phosphorylation relieves the suppression of TSC2 on mTOR-C1, promoting cell proliferation .
Oncogenic Activity
PIM2’s oncogenic activity is significant in hematologic malignancies, particularly multiple myeloma. Its expression is highly elevated in MM cells, and it is required for MM cell proliferation . Functional interference of PIM2 activity, either by short hairpin RNAs or by selective small-molecule inhibitors, leads to significant inhibition of MM cell proliferation .
Therapeutic Potential
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
