PIH1D2 Human

PIH1 Domain Containing 2 Human Recombinant
Cat. No.
BT4297
Source
Escherichia Coli.
Synonyms
PIH1 Domain Containing 2, PIH1D2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

PIH1D2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 338 amino acids (1-315 a.a) and having a molecular mass of 38.3kDa.
PIH1D2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PIH1 Domain Containing 2, also known as PIH1D2, is a member of the PIH1 family. This protein was present in the common ancestor of chordates. There are 45 species without an ortholog for PIH1D2. Additionally, no disorders have been associated with the PIH1D2 gene.
Description
Recombinant human PIH1D2 protein was produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 338 amino acids (amino acids 1-315) with a molecular weight of 38.3 kDa. The PIH1D2 protein has a 23 amino acid His-tag fused to the N-terminus. Purification is carried out using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The PIH1D2 protein solution has a concentration of 0.25 mg/ml and contains phosphate buffered saline (pH 7.4), 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. To ensure stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
PIH1 Domain Containing 2, PIH1D2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMETSSKG LLTQVTQFWN LLDDLAQSDP EGYEKFIQQQ LKEGKQLCAA PEPQLCLQTR ILKPKEKILF INLCQWTRIP APQSTTHPVP LTVGKPEDTT EISDAYTVID VAYNPDVLHA AEKDQVKKNQ LIQMAMKCIE EKFQFTLSHS YHITKFRIKG SIQRMKQNLM GIQTDSIDLR EKMRRELTLG QIRSSTMSNP DHFPQLLLPK DQVSGKAVCL IEEISSTEIQ VEMKMPAYEL KIVHDHSEKP LKIELKVELP GINSVSLCDL SVSEDDLLIE VSEKYRLHLN LPKLIDTEMT TAKFIKEKST LIITMPLV.

Product Science Overview

Gene and Protein Information
  • Gene Symbol: PIH1D2
  • Protein Name: PIH1 Domain Containing 2
  • Aliases: DNAAF15, Dynein Axonemal Assembly Factor 15
  • Species: Human
Function and Importance

PIH1D2 is predicted to be involved in the assembly of box C/D snoRNPs and rRNA processing . The protein encoded by this gene is part of the R2TP complex and ribonucleoprotein complex, which are essential for the proper functioning of cellular processes . Additionally, PIH1D2 has been associated with small GTPase binding activity .

Associated Diseases

Mutations or dysregulation of the PIH1D2 gene have been linked to several diseases, including:

  • Pyruvate Dehydrogenase E2 Deficiency
  • 46,XX Sex Reversal 3
Recombinant Protein

Recombinant human PIH1D2 protein is often used in research to study its function and interactions. The recombinant protein is typically expressed in E. coli and purified for various applications .

Applications in Research

The recombinant PIH1D2 protein is used in various research applications, including:

  • Protein-Protein Interaction Studies: To understand the interactions between PIH1D2 and other proteins.
  • Functional Assays: To study the role of PIH1D2 in cellular processes.
  • Disease Models: To investigate the impact of PIH1D2 mutations on disease development .
Storage and Stability

Recombinant PIH1D2 protein should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain protein stability .

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