PIGR Human
Sf9, Baculovirus cells.
Polymeric immunoglobulin receptor, PIGR, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, polymeric immunoglobulin receptor precursor.
Greater than 95.0% as determined by SDS-PAGE.
Description
PIGR Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 629 amino acids (19-638 aa) and having a molecular mass of 68.9 kDa.
PIGR is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
The polymeric immunoglobulin receptor (PIGR) plays a crucial role in the transcytosis of dimeric IgA and immune molecules. It binds to polymeric IgA and IgM on the basolateral surface of epithelial cells, facilitating their transport across the cell and subsequent secretion at the apical surface. The expression of PIGR is regulated by various factors, including cytokines, hormones, and pathogenic stimuli. Notably, pro-inflammatory cytokines like IL-1, IL-4, TNF-α, and IFN-γ significantly influence its expression.
This product consists of human PIGR, produced in Sf9 Baculovirus cells. It is a single, glycosylated polypeptide chain with a molecular mass of 68.9 kDa. The protein comprises 629 amino acids (19-638 aa) and includes a 6 amino acid His tag at the C-terminus. Purification is achieved through proprietary chromatographic techniques.
The PIGR protein solution is provided at a concentration of 1 mg/ml. It is formulated in Phosphate-Buffered Saline (pH 7.4) and contains 10% glycerol.
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. It's important to avoid repeated freeze-thaw cycles to maintain product integrity.
The purity of the PIGR protein is greater than 95.0%, as determined by SDS-PAGE analysis.
The biological activity of PIGR is evaluated through a functional ELISA using human IgM, assessing its binding ability. The ED50 range is approximately 10 ug/ml.
Polymeric immunoglobulin receptor, PIGR, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, polymeric immunoglobulin receptor precursor.
Sf9, Baculovirus cells.
ADLKSPIFGP EEVNSVEGNS VSITCYYPPT SVNRHTRKYW CRQGARGGCI TLISSEGYVS SKYAGRANLT NFPENGTFVV NIAQLSQDDS GRYKCGLGIN SRGLSFDVSL EVSQGPGLLN DTKVYTVDLG RTVTINCPFK TENAQKRKSL YKQIGLYPVL VIDSSGYVNP NYTGRIRLDI QGTGQLLFSV VINQLRLSDA GQYLCQAGDD SNSNKKNADL QVLKPEPELV YEDLRGSVTF
HCALGPEVAN VAKFLCRQSS GENCDVVVNT LGKRAPAFEG RILLNPQDKD GSFSVVITGL RKEDAGRYLC GAHSDGQLQE GSPIQAWQLF VNEESTIPRS PTVVKGVAGG SVAVLCPYNR KESKSIKYWC LWEGAQNGRC PLLVDSEGWV KAQYEGRLSL LEEPGNGTFT VILNQLTSRD AGFYWCLTNG DTLWRTTVEI KIIEGEPNLK VPGNVTAVLG ETLKVPCHFP CKFSSYEKYW
CKWNNTGCQA LPSQDEGPSK AFVNCDENSR LVSLTLNLVT RADEGWYWCG VKQGHFYGET AAVYVAVEER KAAGSRDVSL AKADAAPDEK VLDSGFREIE NKAIQDPRLF AEEKAVADTR DQADGSRASV DSGSSEEQGG SSRHHHHHH
Product Science Overview
The pIgR is a member of the immunoglobulin superfamily and consists of several key regions:
- Extracellular Region: This region contains five immunoglobulin-like domains that are homologous to the variable regions of immunoglobulins .
- Transmembrane Region: This region anchors the receptor to the cell membrane and also shows homology to certain immunoglobulin variable regions .
- Intracellular Domain: This domain is involved in the intracellular signaling and trafficking of the receptor .
The primary function of pIgR is to mediate the transcytosis of polymeric immunoglobulins, specifically dimeric IgA (dIgA) and pentameric IgM (pIgM), across mucosal epithelial cells . This process is essential for the immune defense of mucosal surfaces, such as those in the gastrointestinal and respiratory tracts .
- Binding: pIgR binds to polymeric immunoglobulins at the basolateral surface of epithelial cells.
- Transcytosis: The receptor-ligand complex is then transported across the cell to the apical surface.
- Secretion: At the apical surface, the extracellular portion of pIgR is cleaved, releasing the immunoglobulin into the mucosal secretions .
The expression and activity of pIgR are regulated by various factors:
- Cytokines: These signaling molecules can upregulate or downregulate the expression of pIgR in response to immune challenges .
- Hormones: Hormonal changes can also influence the expression levels of pIgR .
- Pathogenic Stimuli: The presence of pathogens can stimulate the increased expression of pIgR to enhance immune defense .
Recombinant human pIgR is used in research to study its role in immune responses and its potential therapeutic applications. Understanding the mechanisms of pIgR-mediated transcytosis can provide insights into mucosal immunity and the development of treatments for infections and inflammatory diseases .
