PICK1 Human

Protein Interacting With PRKCA 1 Human Recombinant
Cat. No.
BT13365
Source
Escherichia Coli.
Synonyms
Protein Interacting With PRKCA 1, PRKCABP, Protein Kinase C-Alpha-Binding Protein, Protein Interacting With C Kinase 1, Protein Kinase C, Alpha Binding Protein, Protein Interacting With PRKCA, PRKCA-Binding Protein, PICK, PRKCA-binding protein.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PICK1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 438 amino acids (1-415 a.a) and having a molecular mass of 49.0kDa. 
PICK1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Protein Interacting with PRKCA 1, also known as PICK1, is a protein that contains a PDZ domain. This domain allows PICK1 to interact with protein kinase C, alpha (PRKCA). PICK1 acts as an adaptor molecule, binding to and regulating the subcellular localization of various membrane proteins. It interacts with several glutamate receptor subtypes, monoamine plasma membrane transporters, and non-voltage-gated sodium channels. By targeting PRKCA to these membrane proteins, PICK1 influences their distribution and function. Additionally, PICK1 has been shown to anchor PRKCA to mitochondria in a ligand-specific manner.
Description
Recombinant human PICK1, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 438 amino acids (specifically, amino acids 1 to 415) and has a molecular weight of 49.0 kDa. The protein includes a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The PICK1 protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer containing phosphate-buffered saline (pH 7.4), 30% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised. It is important to avoid repeated cycles of freezing and thawing.
Purity
The purity of the PICK1 protein is determined by SDS-PAGE analysis and is greater than 85.0%.
Synonyms
Protein Interacting With PRKCA 1, PRKCABP, Protein Kinase C-Alpha-Binding Protein, Protein Interacting With C Kinase 1, Protein Kinase C, Alpha Binding Protein, Protein Interacting With PRKCA, PRKCA-Binding Protein, PICK, PRKCA-binding protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMFADLDY DIEEDKLGIP TVPGKVTLQK DAQNLIGISI GGGAQYCPCL YIVQVFDNTP AALDGTVAAG DEITGVNGRS IKGKTKVEVA KMIQEVKGEV TIHYNKLQAD PKQGMSLDIV LKKVKHRLVE NMSSGTADAL GLSRAILCND GLVKRLEELE RTAELYKGMT EHTKNLLRAF YELSQTHRAF GDVFSVIGVR EPQPAASEAF VKFADAHRSI EKFGIRLLKT IKPMLTDLNT YLNKAIPDTR LTIKKYLDVK FEYLSYCLKV KEMDDEEYSC IALGEPLYRV STGNYEYRLI LRCRQEARAR FSQMRKDVLE KMELLDQKHV QDIVFQLQRL VSTMSKYYND CYAVLRDADV FPIEVDLAHT TLAYGLNQEE FTDGEEEEEE EDTAAGEPSR DTRGAAGPLD KGGSWCDS.

Product Science Overview

Gene and Protein Information
  • Gene Name: PICK1 (Protein Interacting with PRKCA 1)
  • Aliases: PRKCABP, Protein Kinase C-Alpha-Binding Protein, Protein Interacting with C Kinase 1
  • Protein Class: Transporters
  • Subcellular Location: Predominantly localized to the cytosol .
Function and Interactions

PICK1 contains a PDZ domain, which allows it to interact with PRKCA. This interaction is crucial for the subcellular localization and function of various membrane proteins. PICK1 has been shown to interact with multiple glutamate receptor subtypes, monoamine plasma membrane transporters, and non-voltage gated sodium channels . These interactions suggest that PICK1 plays a significant role in regulating the distribution and function of these membrane proteins.

Additionally, PICK1 is involved in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. It also plays a role in actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex, which is linked to neuronal morphology regulation and AMPA receptor endocytosis .

Expression and Localization

PICK1 is expressed in various tissues, with the highest expression observed in the endocrine tissues and the central nervous system (CNS). It is also found in the cerebral cortex, cerebellum, basal ganglia, hypothalamus, midbrain, amygdala, choroid plexus, hippocampal formation, spinal cord, retina, thyroid gland, parathyroid gland, adrenal gland, pituitary gland, lung, salivary gland, esophagus, tongue, stomach, duodenum, small intestine, colon, rectum, liver, gallbladder, pancreas, kidney, urinary bladder, testis, epididymis, seminal vesicle, prostate, vagina, ovary, fallopian tube, endometrium, cervix, placenta, breast, heart muscle, smooth muscle, skeletal muscle, adipose tissue, skin, appendix, spleen, lymph node, tonsil, bone marrow, and thymus .

Clinical Significance

Mutations or dysregulation of the PICK1 gene have been associated with various diseases, including male infertility due to globozoospermia and spermatogenic failure 6 . The protein’s role in synaptic plasticity and neuronal morphology also suggests potential implications in neurological disorders.

Research and Applications

PICK1 is a subject of ongoing research due to its involvement in critical cellular processes and its potential implications in various diseases. Understanding the interactions and functions of PICK1 can provide insights into the development of therapeutic strategies for conditions associated with its dysregulation.

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