Phospholipase A2

Phospholipase A2 is a hydrolytic enzyme that specifically targets the sn-2 acyl bond of phospholipids at the lipid-water interface . This enzyme is a 14-16 kDa glycoprotein, consisting of 134 amino acids and a single carbohydrate side chain at the residue Asn13 . The recombinant form of this protein is typically produced in Escherichia coli (E. coli) and includes a 6xHis tag at the C-terminus for purification purposes .

Bee venom phospholipase A2 (BV-PLA2) is the main allergen responsible for bee sting allergies . It can elicit both IgE-mediated allergic reactions and normal immune responses, often associated with high-affinity IgG4 anti-PLA antibodies . BV-PLA2 presents three peptide and one glycopeptide T cell epitopes, which are recognized by both allergic and non-allergic individuals sensitized to bee venom .

The recombinant form of Phospholipase A2 P00630 is used extensively in laboratory research. It is purified using proprietary chromatographic techniques to achieve a purity level of over 90%, as determined by SDS-PAGE . This protein is stable at 4°C for one week but should be stored below -18°C to prevent freeze-thaw cycles .

Phospholipase A2 P00630 is intended for laboratory research use only and is not suitable for use as drugs, agricultural or pesticidal products, food additives, or household chemicals . Proper safety measures should be taken when handling this protein to avoid potential allergic reactions.