Introduction
Pleckstrin homology-like domain family A member 2 (PHLDA2) is a cytoplasmic protein that plays a role in fetal and placental growth. It functions as an apoptosis-related protein and a negative growth regulator, with expression observed during normal human development. PHLDA2 exhibits imprinting in placenta, liver, and fetal tissues during embryogenesis, disappearing after development is complete. Located within the imprinted gene domain of 11p15.5, the PHLDA2 gene is among several genes considered vital for tumor suppression. Alterations in this region might be connected to conditions such as Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and cancers of the lung, ovary, and breast. PHLDA2 expression is detected in the placenta (present in all villous cytotrophoblast cells), adult prostate gland, adult brain, and cell lines of neuroblastoma, medullablastoma, and glioblastoma. Low levels of expression are also observed in the adult liver and lung, as well as in the fetal liver.
Description
Recombinant human PHLDA2, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 172 amino acids (residues 1-152) with a molecular weight of 19.2 kDa. A 20 amino acid His Tag is fused to the N-terminus of PHLDA2. The protein is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
PHLDA2 is supplied in a solution at a concentration of 0.5 mg/ml. The solution also contains 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
While PHLDA2 Human Recombinant remains stable at 4°C for a week, it is recommended to store it at -18°C or below. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is determined to be greater than 85% by SDS-PAGE analysis.
Synonyms
Pleckstrin homology-like domain family A member 2, Imprinted in placenta and liver protein, Tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein, Tumor-suppressing STF cDNA 3 protein, Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein, p17-Beckwith-Wiedemann region 1 C, PHLDA2, BWR1C, HLDA2, IPL, TSSC3, BRW1C.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MKSPDEVLRE GELEKRSDSL FQLWKKKRGV LTSDRLSLFP ASPRARPKEL RFHSILKVDC VERTGKYVYF TIVTTDHKEI DFRCAGESCW NAAIALALID FQNRRALQDF RSRQERTAPA APAEDAVAAA AAAPSEPSEP SRPSPQPKPR TP.
