PhI p 5

Phl p 5.0203 is a glycosylated polypeptide chain with a calculated molecular mass of 27,582 Daltons . The recombinant version of this allergen is produced in Sf9 insect cells using a baculovirus expression system . The protein is expressed with a 10xHis tag at the N-terminus, which facilitates its purification through proprietary chromatographic techniques .

The recombinant Phl p 5.0203 allergen is characterized by its high purity, typically greater than 80% as determined by SDS-PAGE . It is also tested for endotoxins using the Limulus Amoebocyte Lysate (LAL) chromogenic endotoxin assay to ensure its safety for research and therapeutic applications .

Phl p 5.0203 is primarily used in allergen-specific immunotherapy (AIT) for treating grass pollen allergies. Studies have shown that prolonged AIT with Phl p 5.0203 can significantly reduce symptoms and improve the quality of life for patients with allergic rhinitis . The allergen is also used in research to study the mechanisms of allergic reactions and to develop new therapeutic approaches.

The recombinant Phl p 5.0203 allergen is typically stored at -70°C or below to maintain its stability and activity . It is formulated in a neutral to slightly alkaline buffer with 20% glycerol as a cryoprotective agent . Repeated freeze/thaw cycles should be avoided to prevent degradation of the protein.