PHF13 Human

PHD Finger Protein 13 Human Recombinant
Cat. No.
BT3605
Source
Escherichia Coli.
Synonyms
PHF5, SPOC1, PHD finger protein 13, Survival time-associated PHD finger protein in ovarian cancer 1, PHF13.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PHF13 Human Recombinant produced in E. coli is a single polypeptide chain containing 323 amino acids (1-300) and having a molecular mass of 36kDa. PHF13 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PHD finger protein 13 (PHF13) plays a crucial role in cell division (mitosis) by ensuring proper chromosome separation and condensation during the early stages. It achieves this by regulating the structure of chromatin, the material that makes up chromosomes.
Description
This product consists of the human PHF13 protein, manufactured in E. coli bacteria. It's a single chain of 323 amino acids (specifically amino acids 1 to 300), resulting in a protein with a molecular weight of 36kDa. For purification and detection purposes, a 23 amino acid His-tag is attached to the protein's N-terminus. The protein is purified using specialized chromatographic methods.
Physical Appearance
A clear solution that has been sterilized through filtration.
Formulation
The PHF13 protein is provided at a concentration of 0.25mg/ml in a solution containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA, and 250mM Imidazole.
Stability
For short-term storage (2-4 weeks), keep the solution at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeatedly freezing and thawing the solution.
Purity
The purity of the PHF13 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
PHF5, SPOC1, PHD finger protein 13, Survival time-associated PHD finger protein in ovarian cancer 1, PHF13.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDSDSCA AAFHPEEYSP SCKRRRTVED FNKFCTFVLA YAGYIPYPKE ELPLRSSPSP ANSTAGTIDS DGWDAGFSDI ASSVPLPVSD RCFSHLQPTL LQRAKPSNFL LDRKKTDKLK KKKKRKRRDS DAPGKEGYRG GLLKLEAADP YVETPTSPTL QDIPQAPSDP CSGWDSDTPS SGSCATVSPD QVKEIKTEGK RTIVRQGKQV VFRDEDSTGN DEDIMVDSDD DSWDLVTCFC MKPFAGRPMI ECNECHTWIH LSCAKIRKSN VPEVFVCQKC RDSKFDIRRS NRSRTGSRKL FLD.

Product Science Overview

Introduction

PHD Finger Protein 13 (PHF13), also known as SPOC1 (Survival time-associated PHD finger protein in ovarian cancer 1), is a member of the plant homeodomain (PHD) finger protein family. These proteins are characterized by the presence of a PHD finger, a specialized zinc finger domain that recognizes and binds to specific histone modifications, playing a crucial role in chromatin remodeling and transcriptional regulation .

Structure and Function

PHF13 contains a PHD finger domain that allows it to interact with histone tails, particularly recognizing unmodified or methylated lysines on histone H3 . This interaction is essential for its role as an “epigenome reader,” mediating the activation or repression of underlying genes . The protein is required for normal chromosome condensation during the early stages of mitosis and for proper chromosome separation during mitosis .

Biological Significance

PHF13 is involved in various cellular processes, including:

  • Chromatin Remodeling: By binding to histone modifications, PHF13 influences the structure of chromatin, thereby regulating gene expression .
  • Transcriptional Regulation: It plays a role in the activation or repression of genes by interacting with other chromatin-modifying proteins and complexes .
  • Cell Cycle Progression: PHF13 is crucial for the proper condensation and separation of chromosomes during mitosis, ensuring accurate cell division .
Recombinant PHF13

Recombinant human PHF13 protein is produced using recombinant DNA technology. It is typically expressed in E. coli and purified using conventional chromatography techniques . The recombinant protein often includes an N-terminal His-tag to facilitate purification and detection. This recombinant form is used in various research applications to study the protein’s function and interactions.

Applications in Research

Recombinant PHF13 is valuable in research for:

  • Studying Chromatin Dynamics: Researchers use recombinant PHF13 to investigate how it interacts with histone modifications and other chromatin-associated proteins .
  • Gene Expression Analysis: By examining the role of PHF13 in transcriptional regulation, scientists can better understand its impact on gene expression patterns .
  • Cancer Research: Given its association with ovarian cancer, PHF13 is studied for its potential role in cancer development and progression .

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